We narrowed to 400 results for: PUR ALPHA-1

1. W118-1_hRSK3(RPS6KA2)_K91A_K444A

   Plasmid

   #180385

   Purpose

   lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   RPS6KA2_K91A/K444A (RPS6KA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   K91A/K444A

   Promoter

   CMV

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cre Reporter

   Plasmid

   #62732

   Purpose

   Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   LoxP-DsRED-STOP-LoxP-eGFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   LoxP sites

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-Alpha

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. hTRAF2T117ApLPCX-HA-Flag/P875

   Plasmid

   #20230

   Depositor

   Martin Dorf

   Article

   Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

   Insert

   TNF receptor-associated factor 2 (TRAF2 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   T117A (a349g in TRAF2 CDS)

   Promoter

   Available Since

   March 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2987

   Plasmid

   #144463

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RORA (RORA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3015

   Plasmid

   #144491

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PPARA (PPARA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2597

   Plasmid

   #142963

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RORA (RORA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2599

   Plasmid

   #144132

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RORA (RORA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2598

   Plasmid

   #144212

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RORA (RORA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2199

   Plasmid

   #141967

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RXRA (RXRA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-APPSw;T719N

    Plasmid

    #177094

    Purpose

    For lentiviral transduction of APPsw; T719N

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    APPsw;T719N (APP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T719N, K651N and M652L (please see depositor comm…

    Promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PKN1 gRNA (BRDN0001162558)

    Plasmid

    #76278

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN1 (PKN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PRKAA1 gRNA (BRDN0001146526)

    Plasmid

    #76253

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAA1 (PRKAA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PKN1 gRNA (BRDN0001162227)

    Plasmid

    #76276

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN1 (PKN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PRKAA1 gRNA (BRDN0001147273)

    Plasmid

    #76254

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAA1 (PRKAA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. PRKAA1 gRNA (BRDN0001149089)

    Plasmid

    #76255

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAA1 (PRKAA1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PKN1 gRNA (BRDN0001149250)

    Plasmid

    #76275

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN1 (PKN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PKN1 gRNA (BRDN0001148556)

    Plasmid

    #76277

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN1 (PKN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hTRAF2K31RpLPCX-HA-Flag/P876

    Plasmid

    #20231

    Depositor

    Martin Dorf

    Article

    Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

    Insert

    TNF receptor-associated factor 2 (TRAF2 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K31R (a92g in the TRAF2 CDS sequence).

    Promoter

    Available Since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

19. hTRAF2pLPCX-HA-Flag/P874

    Plasmid

    #20229

    Depositor

    Martin Dorf

    Article

    Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

    Insert

    TNF receptor-associated factor 2 (TRAF2 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    unmutated NM_021138.3. L replaces M at beginning…

    Promoter

    Available Since

    March 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEFIRES-P-mCherry-HPos

    Plasmid

    #87159

    Purpose

    Fluorescent marker of lipid droplets (LDs)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    HPos
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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