We narrowed to 604 results for: SaCas9

1. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

   Plasmid

   #177344

   Purpose

   AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   dead hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3x GCN4 peptide, 3xHA, and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   Tetracycline-dependent promoters

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV-CMV-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

   Plasmid

   #177342

   Purpose

   AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   dead hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3x GCN4 peptide, 3xHA, and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CMV

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBK1305-AAV-EFSNC-dSaCas9-HP1a(72-177)

   Plasmid

   #223150

   Purpose

   Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1a (CBX5 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 72-177

   Promoter

   EF1a

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)

   Plasmid

   #223152

   Purpose

   Expression of truncated HP1b with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1b (CBX1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 79-173

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

   Plasmid

   #223163

   Purpose

   2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-miR122 TS

   Plasmid

   #209783

   Purpose

   Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter, incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

   Depositor

   Yuxuan Guo

   Article

   Yang et al Circulation. 2024 May 28;149(22):1778-17

   Insert

   miR122 ts
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   cTNT

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2)

   Plasmid

   #205417

   Purpose

   Mutagenesis of Ntsr1, second guide

   Depositor

   Larry Zweifel

   Insert

   Ntsr1 (Ntsr1 Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

   Plasmid

   #121811

   Purpose

   pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

   Plasmid

   #107321

   Purpose

   Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-SaCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1333S) is fused to SaCas9

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid

    #134470

    Purpose

    Human expression vector for SaCas9-HF variant.

    Depositor

    Zongli Zheng

    Article

    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

    Insert

    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    

    Use

    CRISPR

    Tags

    SV40NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    R245A, N413A, N419A, R654A

    Promoter

    CAG

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJEP313-pAAV-CMV-SaCas9-DIO-pA

    Plasmid

    #113690

    Purpose

    A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9 (NEWENTRY )
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Cytomegalo Virus(CMV)

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJEP304-pAAV-EFS-dSaCas9-VP64-pA

    Plasmid

    #113679

    Purpose

    A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    NLS and VP64

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

    Plasmid

    #78607

    Purpose

    A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short; U6

    Available since

    Sept. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pZac2.1 SV40-CMV-SaCas9-3xNLS

    Plasmid

    #78601

    Purpose

    AAV vector containing SaCas9

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    SV40-CMV-SaCas9-3xNLS
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    SV40, CMV promoters

    Available since

    Dec. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pSaCas9-sgRNA-Tetra-com vector

    Plasmid

    #131227

    Purpose

    For expressing gRNA, whose Tetraloop is replaced with a com aptamer

    Depositor

    Baisong Lu

    Article

    Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

    Insert

    vector for com modified sgRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

    Plasmid

    #113677

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

    Plasmid

    #78606

    Purpose

    pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    173CMV, U6

    Available since

    Dec. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

    Plasmid

    #113681

    Purpose

    A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJEP303-pAAV-CMV-dSaCas9-VP64-pA

    Plasmid

    #113678

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    NLS and VP64

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge