We narrowed to 2,317 results for: TOX

1. pHBSTC146

   Plasmid

   #124357

   Purpose

   Plasmid expressing inactive N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin

   Depositor

   Hideo Iwai

   Article

   Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

   Insert

   gp41-1 N-intein (C1G) and C-intein (bicistronic)
   

   Use

   Tags

   CcdA-C (SFAD)

   Expression

   Bacterial

   Mutation

   C1G

   Promoter

   STC

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCVD630

   Plasmid

   #66163

   Purpose

   cloning of ace gene

   Depositor

   James Kaper

   Article

   Trucksis et al Proc Natl Acad Sci U S A. 1993 Jun 1;90(

   Insert

   ace
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Unknown

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pBHSTC97

   Plasmid

   #124352

   Purpose

   Plasmid expressing NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin

   Depositor

   Hideo Iwai

   Article

   Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

   Insert

   NpuDnaBΔ283 mini-intein
   

   Use

   Tags

   CcdA-C (SFAD)

   Expression

   Bacterial

   Mutation

   Deletion of 283-residue endonuclease domain

   Promoter

   STC

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHBSTC159

   Plasmid

   #124353

   Purpose

   Plasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxin

   Depositor

   Hideo Iwai

   Article

   Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb

   Insert

   NpuDnaBΔ283 (C1G) mini-intein
   

   Use

   Tags

   CcdA-C (SFAD)

   Expression

   Bacterial

   Mutation

   Deletion of 283-residue endonuclease domain, C1G

   Promoter

   STC

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKS1

   Plasmid

   #84079

   Purpose

   Expresses the non-toxic fragment C of tetanus toxin

   Depositor

   Neil Fairweather

   Article

   Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

   Insert

   Tetanus neurotoxin fragment C
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJB37

   Plasmid

   #202602

   Purpose

   Arabinose inducible expression vector expressing retron Rcat-Sen2

   Depositor

   Athanasios Typas

   Article

   Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

   Insert

   RcaT-Sen2 toxin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTV001

   Plasmid

   #130265

   Purpose

   EatA expression plasmid containing 8His encoding region in permissive site of eatA gene

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

   Insert

   eatA::His8
   

   Use

   Tags

   polyhistidine (internal)

   Expression

   Bacterial

   Mutation

   8His polyhistidine encoding region and XhoI site …

   Promoter

   araBAD

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSR0

   Plasmid

   #161984

   Purpose

   Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

   Depositor

   Olga Soutourina

   Article

   Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSR

   Plasmid

   #161983

   Purpose

   Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

   Depositor

   Olga Soutourina

   Article

   Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLinker-AID-3xHA-DHFR-LoxP

    Plasmid

    #86669

    Purpose

    for generation of AID strains using CRISPR tagging technology

    Depositor

    David Sibley

    Article

    Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

    Insert

    Linker-AID-3xHA-HXGPRT 3'UTR
    

    Use

    Expression in toxoplasma gondii

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLinker-AID-Ty-HXGPRT-LoxP

    Plasmid

    #86667

    Purpose

    for generation of AID strains using CRISPR tagging technology

    Depositor

    David Sibley

    Article

    Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

    Insert

    Linker-AID-Ty-HGPRT 3'UTR
    

    Use

    Expression in toxoplasma gondii

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-DHFR

    Plasmid

    #80329

    Purpose

    Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Insert

    DHFR-TSc3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    DHFR-TS 5' UTR

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLoxP-DHFR-mCherry

    Plasmid

    #70147

    Purpose

    Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion

    Depositor

    David Sibley

    Article

    Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/

    Insert

    a selectable dihydrofolate reductase-thymidylate synthase marker
    

    Use

    Expression in toxoplasma gondii

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    DHFR 5' UTR

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. CRT-GFP pcDNA3.1

    Plasmid

    #80978

    Purpose

    mammalian expression of CRT-GFP

    Depositor

    Peter Steyger

    Article

    Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

    Insert

    CRT (Calr Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    no KDEL

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pTW5126

    Plasmid

    #129732

    Purpose

    polyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA protein

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

    Insert

    eatA-DUF::His8
    

    Use

    Tags

    9His tag

    Expression

    Bacterial

    Mutation

    replaces the domain of unknown function correspon…

    Promoter

    araBAD

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAT-YFP-TetR

    Plasmid

    #59018

    Purpose

    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii

    Depositor

    David Sibley

    Article

    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

    Insert

    YFP-TetR
    

    Use

    Toxoplasma expression

    Tags

    Expression

    Mutation

    Promoter

    alpha-tubulin

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid

    #171090

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

    Expression

    Mutation

    D9A, H599A

    Promoter

    TUB1 (Toxoplasma gondii), transcriptionally linke…

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLinker-2xTy-HXGPRT-LoxP

    Plasmid

    #86664

    Purpose

    CRISPR tagging with 2xTy in Toxoplamsa gondii

    Depositor

    David Sibley

    Insert

    Linker-2xTy-HXGPRT 3'UTR
    

    Use

    Expression in toxoplasma gondii

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCVD002

    Plasmid

    #63688

    Purpose

    for use in sequencing and mapping of the V. cholerae enterotoxin genes

    Depositor

    James Kaper

    Article

    Lockman et al J Biol Chem. 1983 Nov 25;258(22):13722-6

    Insert

    enterotoxin genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pccGFPKAN

    Plasmid

    #73649

    Purpose

    Fluorescent reporter for transmembrane protein dimerization.

    Depositor

    Alessandro Senes

    Article

    Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge