Addgene

We narrowed to 2,752 results for: fas

Showing: 241 - 260 of 2752 results

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pAG500-FKBP-N-frFAST

Plasmid

#160060

Purpose

Expresses FKBP-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FKBP-N-frFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac K-M33-Flag

Plasmid

#1993

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

M33 (Cbx2 Mouse)

Use

Tags

Flag

Expression

Insect

Mutation

Kozak sequence added before ATG

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-RS06

Plasmid

#52647

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS06

Use

Tags

Expression

Mammalian

Mutation

M374Q mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-RS14

Plasmid

#52651

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS14

Use

Tags

Expression

Mammalian

Mutation

deleted G47 from GCaMP3

Promoter

T7

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pGBT9-chNfasc (+RSLE)

Plasmid

#89418

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

NFASC (NFASC Chicken)

Use

Tags

GAL4 DBD

Expression

Yeast

Mutation

Promoter

ADH1

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pfast bac Rbx-HA

Plasmid

#51893

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Rbx1 (RBX1 Human)

Use

Tags

HA

Expression

Mutation

Promoter

polyhedrin promoter

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pFastBac His HA GNU WT

Plasmid

#112994

Purpose

Baculovirus expression of His/HA-tagged Drosophila gnu

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

gnu (gnu Fly)

Use

Tags

His/HA

Expression

Insect

Mutation

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-RS12

Plasmid

#52650

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS12

Use

Tags

Expression

Mammalian

Mutation

deleted R40/R41/K42 from GCaMP3

Promoter

T7

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pFastBac-LacI-LAP

Plasmid

#114034

Purpose

Production of baculovirus with LacI-LAP

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP

Use

Tags

Expression

Insect

Mutation

none

Promoter

CMV

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac Mel18 flag

Plasmid

#1969

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Mel18 (Pcgf2 Mouse)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pFasBac+GFP-CAMSAP2 CC

Plasmid

#59040

Purpose

for baculovirus expression of CAMSAP2 CC in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 CC domain (CAMSAP2 Human)

Use

Tags

His and eGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-EF09

Plasmid

#52640

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF09

Use

Tags

Expression

Mammalian

Mutation

D366N mutation from GCaMP3 sequence

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
pFastBac-hDcr-K70A.

Plasmid

#89145

Purpose

Expression of helicase mutant of human Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

hDicer (DICER1 Human)

Use

Tags

His6-tev

Expression

Insect

Mutation

K70A

Promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pFasBac+GFP-CAMSAP3 CC

Plasmid

#59042

Purpose

for baculovirus expression of mouse CAMSAP3 CC in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP3 CC domain (Camsap3 Mouse)

Use

Tags

His and eGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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FASTK gRNA (BRDN0001144738)

Plasmid

#77876

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTK (FASTK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FASTK gRNA (BRDN0001145485)

Plasmid

#77877

Purpose

3rd generation lentiviral gRNA plasmid targeting human FASTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FASTK (FASTK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-EF15

Plasmid

#52641

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF15

Use

Tags

Expression

Mammalian

Mutation

D431N/D433N/D435N mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
OMM-short-CFAST10

Plasmid

#233597

Purpose

Expression of CFAST10 on the OMM membrane after a short linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

CFAST10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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ER-long-RspA-NFAST

Plasmid

#233600

Purpose

Expression of RspA-NFAST on the ER membrane after a 55 aminoacid linker

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-NFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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STIM1-RspA-NFAST

Plasmid

#233606

Purpose

Expression of hSTIM1-RspA-NFAST

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

STIM1 (STIM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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