We narrowed to 674 results for: pet-28

1. pHis-zfCSF3b

   Plasmid

   #65613

   Purpose

   express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

   Depositor

   Petr Bartunek

   Article

   Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

   Insert

   Csf3b (csf3b Zebrafish)
   

   Tags

   (His)6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pN-PITCh-GFP

   Plasmid

   #127888

   Purpose

   Provides the repair template with PSMD2 microhomology on each end for PSMD2 N-ter GFP tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   Puro-GFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pN-PITCh-mCherry

   Plasmid

   #127887

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter mCherry tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   Puro-mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pN-PITCh-HBTH

   Plasmid

   #127883

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-HBTH
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pN-PITCh-HF

   Plasmid

   #127882

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-H3F
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN-PITCh-H3FAID

   Plasmid

   #127884

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-H3F-AID
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. dFP

   Plasmid

   #120542

   Purpose

   Expresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.

   Depositor

   Brian Chow

   Article

   Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

   Insert

   de novo fluorescence-activating protein (dFP)
   

   Tags

   6xHis - TEV cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSKB2-TRexR90D

   Plasmid

   #31350

   Depositor

   Clara Kielkopf

   Article

   McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

   Insert

   Thermus aquaticus Redox Sensing Repressor (R90D)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Arginine 90 to Aspartic Acid

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pISH-H2-Q1-alpha3-domain

    Plasmid

    #15863

    Depositor

    Peter Mombaerts

    Article

    Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

    Insert

    H2-Q1 (H2-Q1 Mouse)
    

    Available Since

    May 6, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pSKB2-TRex

    Plasmid

    #31349

    Depositor

    Clara Kielkopf

    Article

    McLaughlin et al Mol Cell. 2010 May 28. 38(4):563-75.

    Insert

    Thermus aquaticus Redox Sensing Repressor
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pISH-bovine-tau

    Plasmid

    #15864

    Depositor

    Peter Mombaerts

    Article

    Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

    Insert

    bovine tau (MAPT )
    

    Available Since

    May 6, 2008

    Availability

    Academic Institutions and Nonprofits only

13. V2rf2-IRES-BL Targeting Vector

    Plasmid

    #15860

    Depositor

    Peter Mombaerts

    Article

    Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

    Insert

    V2rf2 (Vmn2r81 Mouse)
    

    Expression

    Bacterial

    Available Since

    May 6, 2008

    Availability

    Academic Institutions and Nonprofits only

14. V2rf2 Southern Blot 5'-external probe

    Plasmid

    #15862

    Depositor

    Peter Mombaerts

    Article

    Ishii et al J Neurosci. 2008 Mar 5. 28(10):2332-41.

    Insert

    V2rf2 (Vmn2r81 Mouse)
    

    Expression

    Bacterial

    Available Since

    May 6, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pT-36

    Plasmid

    #16252

    Depositor

    Yoshiko Kikuchi

    Article

    Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

    Insert

    CDC3 (CDC3 Budding Yeast)
    

    Tags

    His and T7

    Expression

    Bacterial

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pHis-zfCSF3b

    Plasmid

    #65613

    Purpose

    express soluble His-tagged zebrafish CSF3b/Gcsfb in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

    Insert

    Csf3b (csf3b Zebrafish)
    

    Tags

    (His)6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pN-PITCh-3XHA

    Plasmid

    #127881

    Purpose

    Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    GFP-Puro-3XHA (PPP2CA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN-PITCh-FAID

    Plasmid

    #127885

    Purpose

    Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID tagging

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Insert

    GFP-Puro-H3F-AID (PPP2CA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. dFP-mini

    Plasmid

    #120543

    Purpose

    Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

    Depositor

    Brian Chow

    Article

    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

    Insert

    de novo fluorescence-activating protein mini (dFP-mini)
    

    Tags

    6xHis - TEV cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-BbsI-PITCh

    Plasmid

    #127875

    Purpose

    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge