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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 24,601 results for: sequence

Showing: 241 - 260 of 24601 results

  15. EK0438 SFFV-mCherry-SGc(s70587)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid
    #191163
    Purpose
    Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).
    Depositor
    Xiaojing Gao
    Article
    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038
    Insert
    mCherry:s70587:EGFP-3xMS2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    SFFV
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191163-plasmid-map-sequence-id-375409

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  16. pRL838

    Plasmid
    #70680
    Purpose
    This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.
    Depositor
    Peter Wolk
    Article
    Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70680-plasmid-map-sequence-id-126382

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  17. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid
    #217635
    Purpose
    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.
    Depositor
    Martin Kampmann
    Article
    Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/
    Insert
    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    EF1alpha and mU6
    Available Since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217635-plasmid-map-sequence-id-449624

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  19. RANLS-LEHD-BNES

    Plasmid
    #60973
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Use
    Tags
    Caspase9 substrate sequence (LEHD) is after NLS s…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 260 of 24601 results