We narrowed to 2,738 results for: GFP reporter gene

1. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

   Plasmid

   #217768

   Purpose

   Fluorescent reporter for ceramide (for stable mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Sleeping beauty transposase-compatible genome ins…

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CA3 domain aa 317-400 plus vector-based linker LE…

   Promoter

   EF-1alpha promoter

   Available since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

   Plasmid

   #217767

   Purpose

   Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Protein purification (6xhis tag, tev site, sumo3 …

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   CA3 domain aa 317-400

   Promoter

   T7 promoter

   Available since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

   Plasmid

   #217766

   Purpose

   Fluorescent reporter for ceramide (for bacterial expression and purification)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Protein purification (6xhis tag, tev site, sumo3 …

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   CA3 domain aa 317-400 plus vector-based linker LE…

   Promoter

   T7 promoter

   Available since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

   Plasmid

   #64314

   Purpose

   chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

   Depositor

   Noriyuki Tsumaki

   Article

   Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

   Inserts

   COL11A2 promoter (COL11A2 Human)
   COL11A2 enhancer (COL11A2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP and IRES-PURO

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

   Plasmid

   #231007

   Purpose

   Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

   Insert

   E4F1 aa220-242 (E4F1 Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+ GFP-CDK5R1 degron (283-307)-IRES-mCherry

   Plasmid

   #231008

   Purpose

   Protein stability reporter construct for CDK5R1 consisting of aa 283-307 for transient overexpression in mammalian cells.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

   Insert

   CDK5R1 aa283-307 (CDK5R1 Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX304-GW-DCK*-IRES-GFP

   Plasmid

   #176291

   Purpose

   Gateway vector for use in generating POI-DCK* fusion reporter

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Insert

   Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S74E R104M D133A

   Promoter

   CMV

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

   Plasmid

   #67979

   Purpose

   Cas9 activity reporter (control) with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

   Plasmid

   #67981

   Purpose

   Cas9 activity reporter (control) with mCherry and GFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX304-DCK*-GW-IRES-GFP

    Plasmid

    #176289

    Purpose

    Gateway vector for use in generating DCK*-POI fusion reporter

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Insert

    Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S74E R104M D133A

    Promoter

    CMV

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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