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paavCAG-JxOFF-post-mGRASP-2A-dTomato

Plasmid

#51906

Purpose

jxOFF-post

Depositor

Jinhyun Kim

Insert

postsynaptic mGRASP

Use

AAV and Cre/Lox

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-SynaptoTAG2

Plasmid

#175275

Purpose

AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

EGFP-Synaptobrevin-2; tdTomato (Vamp2 )

Use

AAV

Tags

EGFP and tdTomato (P2A cleavage)

Promoter

Synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Sico-Red

Plasmid

#84882

Purpose

Cre-inducible shRNA

Depositor

Eun Mi Hwang

Article

Jung et al Exp Neurobiol. 2016 Jun;25(3):120-9. doi

Insert

Sico-Red

Use

AAV

Tags

mCherry

Promoter

EF1a and CMV

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_BiVIPe4_dTomato_nlsdTomato

Plasmid

#213855

Purpose

AAV construct expressing dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe4_dTomato_nlsdTomato

Plasmid

#213936

Purpose

AAV construct expressing dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-mCherry-WPRE-pA

Plasmid

#37119

Purpose

Expresses mCherry in cells lacking Cre (Cre-Off)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

mCherry

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pOTTC293 - pAAV EF1a V5-synuclein (WT)

Plasmid

#60057

Purpose

An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

alpha-synuclein (SNCA Human, human)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-Flpo

Plasmid

#174378

Purpose

also known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Has Service

AAV Retrograde

Insert

FLPo recombinase

Use

AAV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

Plasmid

#207878

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Article

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-GFP-IRES-tTA

Plasmid

#118026

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-fDIO-GFP-IRES-tTA

Use

AAV

Mutation

See depositor comments below

Promoter

TRE

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

Plasmid

#210391

Purpose

AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)

Depositor

Benjamin Kleinstiver

Article

Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:

Insert

AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CamKII promoter for Cre, human U6 promoter for Sp…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DIO-hM4Di-mCherry

Plasmid

#81008

Purpose

Cre-dependent expression of hM4Di-mCherry under CBA promoter

Depositor

Bernardo Sabatini

Article

Hou et al Cell. 2016 Sep 22;167(1):73-86.e12. doi:

Insert

hM4D(Gi)-mCherry

Use

AAV

Tags

mCherry

Promoter

CBA

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-Cyto-TurboID

Plasmid

#149414

Purpose

Hepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

Cyto-TurboID

Use

AAV

Tags

V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-EGFP-Kir4.1

Plasmid

#52874

Purpose

Express EGFP-Kir4.1 fusion protein in astrocytes

Depositor

Baljit Khakh

Article

Tong et al Nat Neurosci. 2014 May;17(5):694-703. do

Insert

Kir4.1 (Kcnj10 Rat)

Use

AAV; Adeno-associated virus ()

Expression

Mammalian

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-EGFP-WPRE-pA

Plasmid

#37091

Purpose

Expresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

d1eGFP

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-cyan pre-eGRASP(p30)

Plasmid

#111586

Purpose

An AAV vector that expresses cyan pre-eGRASP(p30) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p30)

Use

AAV

Promoter

CaMKIIa

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV

Plasmid

#91719

Purpose

AAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2

Depositor

Ryohei Yasuda

Article

Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

Insert

mEGFP-P2A-paAIP2(R5AR6A)

Use

AAV

Promoter

CaMKII 1.3

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-Flex-TC66T

Plasmid

#48331

Purpose

Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA

Depositor

Liqun Luo

Article

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

TVA receptor mCherry fusion with the 66T mutation

Use

AAV and Cre/Lox

Tags

mCherry fusion to TVA receptor

Expression

Mammalian

Mutation

66T

Promoter

CAG

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-mTubb3

Plasmid

#87116

Purpose

AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA

Use

AAV and CRISPR

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI

Plasmid

#189924

Purpose

AAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e V106W

Use

AAV

Mutation

SaCas9 D10A, TadA ABE8e V106W

Promoter

EFS

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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