We narrowed to 43,183 results for: cha

1. CPH3566 (pDEST15_Spt6 1223-1451)

   Plasmid

   #91814

   Purpose

   Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deleted residues 1-1222

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CPH1141 (pET151_Spt6 1247-1451)

   Plasmid

   #91815

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   deleted residues 1-1246

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

   Plasmid

   #115432

   Purpose

   Vector for expressing RPL28 with mutagenized exon2

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   exon 2 deleted, with MfeI / NheI cloning site

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)

   Plasmid

   #106461

   Purpose

   Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W
   

   Use

   Tags

   PA

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)

   Plasmid

   #106470

   Purpose

   Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

   Depositor

   Daniel Klionsky

   Article

   Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

   Insert

   YBR139W
   

   Use

   Tags

   PA

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   YBR139W

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSP64-hERG1a D540K

   Plasmid

   #53057

   Purpose

   Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   changed Aspartate 540 to Lysine

   Promoter

   SP6

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRES:hTRAAK(W262S)

   Plasmid

   #133078

   Purpose

   Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES:hTRAAK(G124I)

   Plasmid

   #133079

   Purpose

   Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G124I

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-mEGFP-CaMKIIa (T286D)

   Plasmid

   #127391

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Aspartic acid

   Promoter

   pCAG

   Available since

   Jan. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-mEGFP-CaMKIIa (T286A)

    Plasmid

    #127390

    Purpose

    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

    Depositor

    Ryohei Yasuda

    Article

    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

    Insert

    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Alanine

    Promoter

    pCAG

    Available since

    Jan. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Kv7.1/pcDNA3.1

    Plasmid

    #111452

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 1 isoform 1 [Homo sapiens] (KCNQ1 Human)
    

    Use

    Tags

    8xHis and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBp-FGFR2b-WT

    Plasmid

    #45698

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIb (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

13. Kv7.4 (1-695)/pcDNA3.1

    Plasmid

    #111453

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    8xHis and EGFP

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-695)

    Promoter

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-Cre-T2A-mRuby2

    Plasmid

    #102989

    Purpose

    Expresses Cre and mRuby2

    Depositor

    Karin Scharffetter-Kochanek

    Article

    Meyer et al PLoS Comput Biol. 2017 Dec 4;13(12):e100

    Inserts

    Cre (site-specific recombinase)
    mRuby2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBp-FGFR2c-WT

    Plasmid

    #45699

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIc (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SNAP-CLC-EGFP

    Plasmid

    #193579

    Purpose

    Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    SNAP-tag and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC8_MF-p10-mCh (VE5626)

    Plasmid

    #139772

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with mCherry cDNA under p10 to monitor infection.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    mCherry fluorescent protein
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PH

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBp-FGFR3c-R248C

    Plasmid

    #45712

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR3 IIIc (FGFR3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    R248C

    Promoter

    Available since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-mScarlet-gamma9

    Plasmid

    #140097

    Purpose

    Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    GNGT2
    

    Use

    Tags

    N-terminal mScarlet tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBp-FGFR2b-K660E

    Plasmid

    #45705

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIb (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K660E

    Promoter

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only