We narrowed to 246,630 results for: des

1. Munc18cwt-pDEST27

   Plasmid

   #85626

   Purpose

   GST tagged Munc18c

   Depositor

   David James

   Article

   Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

   Insert

   Munc18c (Stxbp3 Mouse)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT-DestFLAGHA_HuC

   Plasmid

   #65759

   Purpose

   constitutively expresses FLAG-HA-tagged HuC in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL3 (ELAVL3 Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pDEST-ERBB3-v1

   Plasmid

   #154893

   Purpose

   Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Insert

   ERBB3 (ERBB3 Human)
   

   Use

   Tags

   Venus fragment 1 (v1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST14-SpyCatcher002 EQ

   Plasmid

   #102830

   Purpose

   Bacterial expression of unreactive version of SpyCatcher002

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002 EQ
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   E77Q mutation prevents isopeptide bond formation

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNR-DEST

   Plasmid

   #90101

   Purpose

   Gateway destination vector featuring nitrate reductase (NR) promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.

   Depositor

   Philip Weyman

   Article

   Bielinski et al PeerJ Preprints. 2017 5:e2907v1

   Type

   Empty backbone

   Use

   Gateway destination vector for protein expression…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pH4-DEST

   Plasmid

   #90099

   Purpose

   Gateway destination vector featuring H4 promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.

   Depositor

   Philip Weyman

   Article

   Bielinski et al PeerJ Preprints. 2017 5:e2907v1

   Type

   Empty backbone

   Use

   Gateway destination vector for protein expression…

   Tags

   Expression

   Mutation

   Promoter

   H4

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMB-PDESr

   Plasmid

   #61207

   Purpose

   Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   AtPDLP1
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   MtBCP1

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-HemmarI2

   Plasmid

   #112815

   Purpose

   gateway cloning destination vector for expressing enhancer driven CD4-IFP2.0-T2A-HO1

   Depositor

   Chun Han

   Article

   Poe et al Genetics. 2018 Nov 30. pii: genetics.118

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFRT-DestFLAGHA_RNAseH1

   Plasmid

   #65783

   Purpose

   constitutively expresses FLAG-HA-tagged RNAseH1 in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   RNaseH1 (RNASEH1 Human, Zebrafish)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST17-UbE2U

    Plasmid

    #15810

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2U (UBE2U Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pMMDS DEST

    Plasmid

    #206264

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Cre-donor, multimate/gateway dest vector (multiba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mApple-Desmoglein1-N-18

    Plasmid

    #54888

    Purpose

    Localization: Desmosomes, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Desmoglein1 (DSG1 Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST47-ARL8B

    Plasmid

    #67467

    Purpose

    Mammalian expression of hArl8b with stop codon (native expression)

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pEF.DEST51-mVenus

    Plasmid

    #154899

    Purpose

    Expresses a fluorescent protein, mVenus

    Depositor

    Johanna Ivaska

    Article

    Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

    Insert

    mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFRT-TODestFLAGHA_HuB

    Plasmid

    #65755

    Purpose

    expresses FLAGHA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Use

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFUSE-DEST_R1R4

    Plasmid

    #97186

    Purpose

    For fusion gene cloning. Recombine Gene A + Gene B into this Gateway Destination Vector

    Depositor

    Kenneth Scott

    Article

    Lu et al Cancer Res. 2017 May 16. pii: canres.274

    Type

    Empty backbone

    Use

    Lentiviral; Gateway

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST17-Tpt1

    Plasmid

    #42571

    Depositor

    Stan Fields, Jay Hesselberth

    Article

    Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

    Insert

    Tpt1
    

    Use

    Tags

    6xHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA Polmyerase

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pDEST_6xUAS:lyn-tagRFPt

    Plasmid

    #192354

    Purpose

    lyn-tagRFPt expression under the UAS promoter

    Depositor

    Darren Gilmour

    Article

    Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

    Insert

    lyn-tagRFPT
    

    Use

    Tags

    tagRFPT

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST17-ALDH1A1

    Plasmid

    #189749

    Purpose

    The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A1 (ALDH1A1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMetOYC-Dest

    Plasmid

    #105082

    Purpose

    Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning

    Depositor

    Christopher Grefen

    Article

    Xing et al Plant Physiol. 2016 Jun;171(2):727-58. d

    Type

    Empty backbone

    Use

    Tags

    Cub-PLV and mOST4

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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