We narrowed to 8,124 results for: golden gate

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pCBLggTerm

Plasmid

#199738

Purpose

Golden Gate entry vector for terminator cloning

Depositor

Kan Wang

Publication

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Expression

Plant

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pZA55

Plasmid

#174768

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E without STOP codon

Use

Unspecified

Mutation

L17E

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA56

Plasmid

#174769

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E/D481V without STOP codon

Use

Unspecified

Mutation

L17E/D481V

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA54

Plasmid

#174767

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V without STOP codon

Use

Unspecified

Mutation

D481V

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pZA53

Plasmid

#174766

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn without STOP codon

Use

Unspecified

Mutation

WT

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1360 - Intron GG3 - smu-1

Plasmid

#159806

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - smu-1

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1358 - Intron GG1 - smu-1

Plasmid

#159804

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - smu-1

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLY134

Plasmid

#130962

Purpose

Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJET-TtrpC

Plasmid

#213467

Purpose

Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Publication

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Terminator of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-PgpdA

Plasmid

#213466

Purpose

Vector containing the gpdA promoter for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Publication

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Promoter of the glyceraldehyde-3-phosphate dehydrogenase

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-PtrpC

Plasmid

#213465

Purpose

Vector containing the TrpC promoter for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Publication

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Promoter of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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Modular BE4max for GG insertion

Plasmid

#198891

Purpose

Easily insert deaminase of choice into BE4max using Golden Gate cloning

Depositor

Reuben Harris

Publication

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

CRISPR associated protein 9 (cas9 Streptococcus pyogenes)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

Plasmid

#178986

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

Plasmid

#178990

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1397-C

Plasmid

#174563

Purpose

mitochondrial-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Publication

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-C

Tags

FLAG

Expression

Plant

Promoter

PcUbi

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1397-N

Plasmid

#174562

Purpose

mitochondrial-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Publication

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-N

Tags

HA

Expression

Plant

Promoter

PcUbi

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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cp-DdCBE G1397-C

Plasmid

#174559

Purpose

Chloroplast-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Publication

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-C

Tags

FLAG

Expression

Plant

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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cp-DdCBE G1397-N

Plasmid

#174558

Purpose

Chloroplast-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Publication

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-N

Tags

HA

Expression

Plant

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pL0-BvCYP76AD1

Plasmid

#162529

Purpose

Beta vulgaris cytochrome P450 76AD1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

Depositor

Sam Brockington

Publication

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

CYP76AD1

Use

Puc19-derived

Expression

Bacterial

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CB0026

Plasmid

#162285

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Publication

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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