We narrowed to 5,064 results for: cre encoding plasmid

1. pMT335-mScarletI

   Plasmid

   #174506

   Purpose

   High-copy number plasmid (pMT335) harboring the mScarletI gene for its cytoplasmic expression, inducible by vanillate (sequence of mScarletI optimized)

   Depositor

   Suliana Manley

   Article

   Mahecic et al Nat Methods. 2022 Sep 8. pii: 10.1038/s4

   Insert

   mScarletI
   

   Use

   Tags

   mScarletI

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

   Plasmid

   #112669

   Purpose

   Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.

   Depositor

   Catherine Verfaillie

   Article

   Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

   Insert

   tdT
   

   Use

   AAV; Donor plasmid for recombinase-mediated casse…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRPF185

   Plasmid

   #106367

   Purpose

   C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

   Depositor

   Neil Fairweather, Robert Fagan

   Article

   Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

   Insert

   Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   codon optimised for C. difficile

   Promoter

   Ptet

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCB320

   Plasmid

   #89359

   Purpose

   3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

   Depositor

   Michael Bassik

   Article

   Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

   Insert

   puromycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5xGAL4-TATA-luciferase

   Plasmid

   #46756

   Purpose

   Luciferase reporter gene for factors with GAL4 DNA binding domains

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   Luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   minimal TATA

   Available since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-RSV-SpCas9

   Plasmid

   #85450

   Purpose

   Express SpCas9 in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pRSV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pRSV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMCB306

   Plasmid

   #89360

   Purpose

   sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

   Depositor

   Michael Bassik

   Article

   Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

   Insert

   GFP, puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. RINS1

   Plasmid

   #107290

   Purpose

   Plasmid for the expression of the RINS1 sensor

   Depositor

   Dmytro Yushchenko

   Article

   Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

   Insert

   RINS1
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKHH030

    Plasmid

    #89358

    Purpose

    hU6 sgRNA cloning vector for CDKO system

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRPF144

    Plasmid

    #106372

    Purpose

    C. difficile constitutive expression system. Shuttle plasmid containing constitutive Pcwp2 -gusA

    Depositor

    Neil Fairweather, Robert Fagan

    Article

    Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

    Insert

    Pcwp2-gusA (gusA )
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    codon optimised for C. difficile

    Promoter

    Pcwp2

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG306-GPD-AVT1 chr I

    Plasmid

    #41898

    Purpose

    Yeast expression of AVT1.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    AVT1 (AVT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. mUAVbla:tetR 5′

    Plasmid

    #149379

    Purpose

    mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Insert

    tetA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGM9121_GFP11

    Plasmid

    #153508

    Purpose

    GFP11 C-terminal Tag Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    GFP11
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CyclinB-GFP

    Plasmid

    #54465

    Purpose

    CyclinB-GFP in pIVT vector for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

    Insert

    CyclinB
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K67E and I150T mutations compared to GenBank refe…

    Promoter

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

16. IL1_1

    Plasmid

    #207623

    Purpose

    Low copy plasmid expressing mTurquoise2 under the TDH3p promoter

    Depositor

    Yvonne Nygård

    Article

    Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGM9121_TwinStrep

    Plasmid

    #153513

    Purpose

    TwinStrep C-terminal Tag Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    TwinStrep
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid

    #224697

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Use

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pRARB.1.0-gDNA

    Plasmid

    #132471

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    RARB (RARB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG306-GPD-VPH2 chr I

    Plasmid

    #41897

    Purpose

    Yeast expression of VPH2.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    VPH2 (VPH2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Feb. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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