We narrowed to 300,363 results for: ins

1. pUASP YFP Rab21 WT

   Plasmid

   #37723

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab21 (Rab21 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Promoter

   GAL4

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pUASp YFP RabX2 WT

   Plasmid

   #37753

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   RabX2 (RabX2 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Promoter

   GAL4

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pUASp YFP RabX2 CA

   Plasmid

   #37770

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   RabX2 (RabX2 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   D66L (constitutive active)

   Promoter

   GAL4

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pUASp YFP RabX5 CA

   Plasmid

   #37779

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   RabX5 (RabX5 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q123L (constitutive active)

   Promoter

   GAL4

   Available Since

   Nov. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pUASp YFP Rab2 WT

   Plasmid

   #37684

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab2 (Rab2 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Promoter

   GAL4

   Available Since

   Nov. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCY 3120-01

   Plasmid

   #36237

   Depositor

   Anne Robinson

   Article

   Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

   Insert

   yECitrine-7Myc
   

   Use

   Yeast cassette for pcr and homologous recombinati…

   Tags

   yEpolylinker-yECitrine-7Myc-kanMX

   Available Since

   Aug. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAraH6HATT

   Plasmid

   #63901

   Purpose

   For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.

   Depositor

   Carlos Barbas

   Article

   Barbas et al Methods, A companion to Methods in Enzym

   Insert

   TT Fab
   

   Tags

   6xHis and HA tag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYMA1_SidK_mCH

   Plasmid

   #184906

   Purpose

   Legionella pneumophila SidK (1-278) - mCherry

   Depositor

   John Rubinstein

   Article

   Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

   Insert

   SidK (1-278) (sidK Legionella pneumophila)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HIV-1 integrase F185K/C280S in pET-15b

   Plasmid

   #61669

   Purpose

   HIV-1 integrase F185K/C280S in pET-15b

   Depositor

   Robert Craigie

   Article

   Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

   Insert

   HIV-1 integrase F185K/C280S
   

   Use

   Synthetic Biology

   Tags

   His

   Promoter

   T7 promotor

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pDonor14-ShHELIX-KanR (CJT70)

    Plasmid

    #181793

    Purpose

    pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Expression

    Bacterial

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYMA3_SidK_GFP

    Plasmid

    #184276

    Purpose

    Legionella pneumophila SidK (1-278) - GFP

    Depositor

    John Rubinstein

    Article

    Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

    Insert

    SidK (1-278) (sidK Legionella pneumophila)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMSP1E1D1

    Plasmid

    #161605

    Purpose

    Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E1D1
    

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDonor14-N7HELIX-KanR

    Plasmid

    #190663

    Purpose

    Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Expression

    Bacterial

    Promoter

    NA

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-HxB.L

    Plasmid

    #65414

    Purpose

    contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

    Depositor

    Harold Erickson

    Article

    Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

    Insert

    TNC large splice variant (TNC Human)
    

    Expression

    Bacterial

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSP1E2D1

    Plasmid

    #161606

    Purpose

    Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP1E2D1
    

    Tags

    7-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1D1; contains repeat of h…

    Promoter

    T7

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDD284

    Plasmid

    #66825

    Purpose

    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pVTU_260_BirA

    Plasmid

    #63682

    Purpose

    Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

    Insert

    BirA
    

    Tags

    6X Histidine and TEV cleavage site

    Expression

    Yeast

    Promoter

    ADH1/yeast

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSC101-GFPmut2-mScarlet-I TAA

    Plasmid

    #208187

    Purpose

    nonsense mistranslation, TAA stop

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Insertion 6TAA*(stop) 7AGT(Ser)

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDD285

    Plasmid

    #66826

    Purpose

    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mKate2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized mKate2

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pBS-HxB.S

    Plasmid

    #65415

    Purpose

    contains complete cDNA for TNC small splice variant flanked by NotI-KpnI

    Depositor

    Harold Erickson

    Article

    Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

    Insert

    tenascin C small splice variant (TNC Human)
    

    Expression

    Bacterial

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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