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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,839 results for: pcr

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Showing: 2601 - 2620 of 4839 results

  1. pDisplay-GRAB_NTS1.0

    Plasmid
    #208670
    Purpose
    Expresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in mammalian cells
    Depositor
    Yulong Li
    Publication
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208670-plasmid-map-sequence-id-412150

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  2. P2RY8-Tango

    Plasmid
    #66477
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P2RY8 (P2RY8 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. MC5R-Tango

    Plasmid
    #66431
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MC5R (MC5R Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. GPR32-Tango

    Plasmid
    #66352
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR32 (GPR32 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66352-plasmid-map-sequence-id-168125

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  5. GPR153-Tango

    Plasmid
    #66329
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR153 (GPR153 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. GAL3-Tango

    Plasmid
    #66290
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAL3 (GALR3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. GPR1-Tango

    Plasmid
    #66300
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPR1 (GPR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. LPAR1-DuET

    Plasmid
    #213329
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    LPAR1 (LPAR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213329-plasmid-map-sequence-id-430863

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  9. F2R-DuET

    Plasmid
    #213234
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    F2R (F2R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213234-plasmid-map-sequence-id-430897

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  10. pcDNA3-PPO-mScarlet

    Plasmid
    #139503
    Purpose
    Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.
    Depositor
    Michael Bruchas
    Publication
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    parapinopsin
    Tags
    mScarlet following Gly-Gly-Ser linker
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139503-plasmid-map-sequence-id-288142

    Enlarge

  11. QRFP-Tango

    Plasmid
    #66491
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    QRFP (QRFPR Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. OPN3-Tango

    Plasmid
    #66459
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OPN3 (OPN3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  16. mutpRL-TK 1x perfect to bulged siRNA

    Plasmid
    #40765
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to bulged target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Dec. 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. mutpRL-TK 1x perfect to seed siRNA

    Plasmid
    #40766
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to seed target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. GRM1-DuET

    Plasmid
    #213300
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GRM1 (GRM1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213300-plasmid-map-sequence-id-430906

    Enlarge

  20. OPN5-Tango

    Plasmid
    #66460
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OPN5 (OPN5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2601 - 2620 of 4839 results