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YIplac204-TDH3p-CDC19-CYC1t

Plasmid

#216122

Purpose

Yeast integrative vector for constitutive expression of CDC19 at the TRP1 locus

Depositor

Yvonne Nygård

Article

Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

Insert

Pyruvate kinase 1

Expression

Yeast

Promoter

TDH3 promoter

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAM72

Plasmid

#227622

Purpose

pETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)

Depositor

Andreas Martin

Article

Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

Inserts

Rpn11 (RPN11 Budding Yeast)
rpn8 (RPN8 Budding Yeast)

Tags

His-Prescission

Expression

Bacterial

Mutation

1-179 and aa 2-239, G77P

Promoter

T7

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgSeLEU2

Plasmid

#224870

Purpose

Disruption of S. eubayanus type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence SeLEU2 (DI49_0736 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTrGCsgScLEU2

Plasmid

#224869

Purpose

Disruption of S. cerevisiae type LEU2 genes

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTrGCas having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBWB507

Plasmid

#209325

Purpose

p15A-tetR-tet-ME-RBS-mRFP-ME-U1-Barcode-U2-dbI terminator-oriT-Cm. Plasmid backbone for fragment expression.

Depositor

Adam Arkin

Article

Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

Insert

mRFP

Expression

Bacterial

Promoter

tet

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgScLEU2

Plasmid

#224868

Purpose

Disruption of S. cerevisiae type LEU2 gene

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-APP-CTF

Plasmid

#181732

Purpose

Galactose inducible expression of APP-CTF

Depositor

Alejandro Chavez

Insert

APP-CTF (APP Human)

Expression

Yeast

Mutation

C-terminal fragment (aa 671-770)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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BcC _pET29b

Plasmid

#215403

Purpose

Expression construct for BcC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

BcC

Expression

Bacterial

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pITESceIcmd

Plasmid

#218288

Purpose

Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

Depositor

Claudia Vickers

Article

McDonnell et al bioRxiv 2024.03.26.586389

Insert

HO(-253, -1)-tSynth3

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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OoH_pET29b

Plasmid

#215424

Purpose

Expression construct for OoH gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

OoH

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Ph-NylC_pET28a

Plasmid

#215425

Purpose

Expression construct for Ph-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Ph-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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ThcCut2 _pET29b

Plasmid

#215430

Purpose

Expression construct for ThcCut2 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

ThcCut2

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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TtA_pET29b

Plasmid

#215432

Purpose

Expression construct for TtA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

TtA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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MsmegA _pET29b

Plasmid

#215411

Purpose

Expression construct for MsmegA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

MsmegA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Nc-NylC_pET28a

Plasmid

#215412

Purpose

Expression construct for Nc-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Nc-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AoC_pET29b

Plasmid

#215402

Purpose

Expression construct for AoC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

AoC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-Snf7-4V5-APEX2-URA

Plasmid

#207065

Purpose

Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Snf7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP2-Mss4D636K-Kina

Plasmid

#202725

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756)(D636K) (MSS4 Budding Yeast)

Expression

Mammalian

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.T02R

Plasmid

#198654

Purpose

Easy-MISE toolkit pEM-plasmid containing CYC1 terminator with IL protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

tCYC1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.T01L

Plasmid

#198653

Purpose

Easy-MISE toolkit pEM-plasmid containing ADH1 terminator with BC protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

tADH1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.P06R

Plasmid

#198652

Purpose

Easy-MISE toolkit pEM-plasmid containing PDA1 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pPDA1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.P06L

Plasmid

#198651

Purpose

Easy-MISE toolkit pEM-plasmid containing PDA1 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pPDA1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.P05R

Plasmid

#198650

Purpose

Easy-MISE toolkit pEM-plasmid containing CYC1 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pCYC1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.P05L

Plasmid

#198649

Purpose

Easy-MISE toolkit pEM-plasmid containing CYC1 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pCYC1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.P04R

Plasmid

#198648

Purpose

Easy-MISE toolkit pEM-plasmid containing TPI1 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pTPI1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.P04L

Plasmid

#198647

Purpose

Easy-MISE toolkit pEM-plasmid containing TPI1 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pTPI1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.P03R

Plasmid

#198646

Purpose

Easy-MISE toolkit pEM-plasmid containing PGK1 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pPGK1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.P03L

Plasmid

#198645

Purpose

Easy-MISE toolkit pEM-plasmid containing PGK1 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pPGK1

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.P02R

Plasmid

#198644

Purpose

Easy-MISE toolkit pEM-plasmid containing ENO2 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pENO2

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.P02L

Plasmid

#198643

Purpose

Easy-MISE toolkit pEM-plasmid containing ENO2 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pENO2

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P01R

Plasmid

#198642

Purpose

Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with FG protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pTDH3

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM.P01L

Plasmid

#198641

Purpose

Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with EF protruding ends

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pTDH3

Use

Synthetic Biology

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pOBD2

Plasmid

#196826

Purpose

Yeast two-hybrid ; Gal4 DNA binding domain

Depositor

Stan Fields

Article

Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

Type

Empty backbone

Tags

Gal4 DNA binding domain

Expression

Yeast

Promoter

ADH

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-Cys-HA-Ubiquitin

Plasmid

#185356

Purpose

In vitro ubiquitination assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Ubiquitin

Tags

GST, HA(x2)

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_32_PyruEth_X2site

Plasmid

#194428

Purpose

Plasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

PyruEth

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_75_Donor_Tag_RPL13A-mTurq2

Plasmid

#194415

Purpose

Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

mTurquoise2 CDS

Use

Synthetic Biology; Used as donor dna in genome in…

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-SpDcp2opt_1-242-E147Q_AD

Plasmid

#148546

Purpose

Bacterial Expression of SpDcp2opt_1-242-E147Q

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

SpDcp2opt_1-242-E147Q (dcp2 Fission Yeast)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pILGFP4D42

Plasmid

#185840

Purpose

Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP10CF71

Plasmid

#185860

Purpose

Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP10CF72 (B9)

Plasmid

#185858

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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