We narrowed to 7,410 results for: 11

1. pEasyG1_nat

   Plasmid

   #184911

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. dSpCas9-VPR

   Plasmid

   #205247

   Purpose

   In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   dSpCas9-VPR
   

   Use

   CRISPR

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DHFR-SpCas9-DHFR

   Plasmid

   #85447

   Purpose

   Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

   Insert

   DHFR-SpCas9-DHFR
   

   Use

   AAV

   Tags

   Destabilized domain (C-terminus version) of E.col…

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-PM-ExRai-CKAR2

   Plasmid

   #236098

   Purpose

   Plasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   Lyn-ExRai-CKAR2
   

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV5B-TGFbeta receptor II wt

   Plasmid

   #11766

   Depositor

   Joan Massague, Jeff Wrana

   Article

   Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

   Insert

   TGFbeta receptor II (TGFBR2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. GFP-Tollip

   Plasmid

   #178050

   Purpose

   GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.

   Depositor

   David Tumbarello

   Article

   Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

   Insert

   Toll-Interacting Protein (TOLLIP Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3 Flag Jnk3a2

   Plasmid

   #13759

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk3a2 (MAPK10 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pCDNA3 Flag Jnk2a2

   Plasmid

   #13755

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk2a2 (MAPK9 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3 Flag Jnk2a1

   Plasmid

   #13754

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk2a1 (MAPK9 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA3 Flag Jnk1a2

    Plasmid

    #13751

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk1a2 (MAPK8 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pEG BacMam C term 3C eGFP His8

    Plasmid

    #160687

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Tags

    3C eGFP His8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpCas9n(BB)-2A-Puro (PX462)

    Plasmid

    #48141

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pWay21-MKP-1 FL

    Plasmid

    #13469

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1 FL (Dusp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-HA-JMJD2B

    Plasmid

    #24181

    Purpose

    Mammalian expression of human JMJD2B with HA tag

    Depositor

    Kristian Helin

    Article

    Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

    Insert

    JMJD2B (KDM4B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FU-tet-o-hc-myc

    Plasmid

    #19775

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    c-myc (MYC Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV sgRNA Expression Plasmid

    Plasmid

    #174540

    Purpose

    Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

    Depositor

    Ormond MacDougald

    Article

    Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMRX-IZ-ANXA11-mRuby3

    Plasmid

    #184894

    Purpose

    Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA11 (ANXA11 Human)
    

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    R230C relative to NP_001148.1

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEG BacMam N term His8 eGFP 3C

    Plasmid

    #160684

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Tags

    His8 eGFP 3C

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Puro-Cas9 donor

    Plasmid

    #58409

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD_FLAG-GFP(Y39TAG)-6His

    Plasmid

    #223510

    Purpose

    Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Insert

    GFP(Y39TAG)
    

    Tags

    6xHis and FLAG

    Expression

    Bacterial

    Mutation

    Y39TAG

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge