We narrowed to 3,960 results for: LYC

1. pDGE501

   Plasmid

   #153274

   Purpose

   M7 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGE499

   Plasmid

   #153272

   Purpose

   M6 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGE498

   Plasmid

   #153271

   Purpose

   M5 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4136457

   Plasmid

   #149537

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGBW-m4046739

   Plasmid

   #145617

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGBW-m4046817

   Plasmid

   #145607

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. FoxFTVC+bpFOG_MRAS-G22V

   Plasmid

   #107521

   Purpose

   Constitutive activation of FGF/MAPK pathway by M-Ras-G22V mutant

   Depositor

   Lionel Christiaen

   Article

   Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

   Insert

   M-Ras protein
   

   Use

   Tags

   Expression

   Mutation

   changed Glycine 22 to Valine

   Promoter

   TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIplac204-pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)

   Plasmid

   #115428

   Purpose

   Expresses a non-glycosylated regulatable secretory cargo for yeast at the TRP1 locus

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p1G108C-GG

   Plasmid

   #85092

   Purpose

   Host vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   DNA polymerase sliding clamp 1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Glycine 108 to Cysteine

   Promoter

   T7 promoter

   Available since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNH (GB0064)

    Plasmid

    #68165

    Purpose

    Provides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlNH promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPUbc (GB0145)

    Plasmid

    #68170

    Purpose

    Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlUbc promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK315

    Plasmid

    #71141

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes histidine-89 to glycine; additional N-ter…

    Promoter

    T7

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2A11 (GB0143)

    Plasmid

    #68189

    Purpose

    Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Sl2A11 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTE8 (GB0144)

    Plasmid

    #68190

    Purpose

    Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlE8 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC-EsaR-D91G/V220A

    Plasmid

    #47648

    Purpose

    pAC-EsaR with a272g and c659t mutations in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-D91G/V220A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 91 to Glycine, and Valine 2…

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

16. HI.HslU(delI)V.RSET.wt(Nde1).107-GG-244

    Plasmid

    #17859

    Depositor

    David McKay

    Article

    Kwon et al J Mol Biol. 2003 Jul 4. 330(2):185-95.

    Insert

    Haemophilus influenzae heat shock loci U and V
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    coding sequence for residues 108-243 of HslU (the…

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sup35 Sc/Ca NM Chimera 4 G/A His7 Cys

    Plasmid

    #15602

    Depositor

    Susan Lindquist

    Article

    Tessier et al Nature. 2007 May 31. 447(7144):556-61.

    Insert

    Sup35 NM (SUP35 Budding Yeast, Candida albicans)
    

    Use

    Tags

    7xHis and Cysteine

    Expression

    Bacterial

    Mutation

    4 G/A. Four glycines mutated to alanines. The pos…

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSUP-(2xCouRS-tRNAopt)

    Plasmid

    #197930

    Purpose

    Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

    Inserts

    CouRS
    tRNA opt
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    glnS' and lambda(ts) and proK

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKC52

    Plasmid

    #226780

    Purpose

    Expresses Drosophila UNC-104(R348G) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (R348G) (unc-104 Fly)
    

    Use

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Arginine 348 to Glycine

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT22-GluCNZ-6His

    Plasmid

    #213504

    Purpose

    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

    Depositor

    John Denu

    Article

    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

    Insert

    Glucanase (glucan endo-1,3-beta-glucosidase)
    

    Use

    Tags

    6His tag

    Expression

    Bacterial

    Mutation

    1-36 Signal Sequence Deletion

    Promoter

    T7

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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