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pRK5_tAKAR4α

Plasmid

#119919

Purpose

Mammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imaging

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKAR4α

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-DBD

Plasmid

#14874

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

DAG binding domain (Prkcb Rat)

Tags

YFP

Expression

Mammalian

Mutation

Y123W

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
Ube2r1

Plasmid

#31426

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (CDC34 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
Ube2s

Plasmid

#31417

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBA7 Human)

Tags

HA

Expression

Yeast

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
Wnt7A STOP

Plasmid

#35877

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7A (WNT7A Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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FLS-PC1

Plasmid

#41520

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Polycystin-1 (PKD1 Human)

Tags

Myc/His

Expression

Mammalian

Mutation

sequence from 4107 - 4303 of PKD1

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 EGFP

Plasmid

#115786

Purpose

Expresses EGFP in mammalian cells

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX3X-RanBP2dFG

Plasmid

#53120

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

RanBP2 fragment (RANBP2 Human)

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2553-2838)

Promoter

tac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pJDC124

Plasmid

#157877

Purpose

mCherry is expressed under the mycobacterial phsp60 heat shock promoter for in vivo and in vitro detection.

Depositor

Jeffrey Cirillo

Article

Kong et al PLoS One. 2016 Mar 2;11(3):e0149972. doi

Insert

mCherry

Expression

Bacterial

Promoter

phsp60

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5B

Plasmid

#35912

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5B (WNT5B Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAMS366-4xCDRE-GFP-PEST

Plasmid

#138658

Purpose

destabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiae

Depositor

Sabrina Büttner

Article

Diessl et al Microbial Cell, 2020: in press

Insert

4xCDRE-CYC1(PM)-yEGFP3-PEST

Use

Yeast reporter plasmid

Promoter

CYC1

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt9A

Plasmid

#35918

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9A (WNT9A Human)

Expression

Mammalian

Mutation

Silent Mutation A (543) --> G

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSCW02

Plasmid

#72301

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Expression

Bacterial

Promoter

lac

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Golgi-Ras-LOCKR-S

Plasmid

#216499

Purpose

FRET-based reporter for Ras activity, Golgi localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Golgi-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 ER-Ras-LOCKR-S

Plasmid

#216500

Purpose

FRET-based reporter for Ras activity, ER localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

ER-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Mito-Ras-LOCKR-S

Plasmid

#216501

Purpose

FRET-based reporter for Ras activity, mitochondria localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Mito-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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Wnt5A STOP

Plasmid

#35874

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Use

Entry vector

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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FLM-PC1

Plasmid

#41567

Depositor

Thomas Weimbs

Article

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

sequence from 4078 - 4303 of PKD1

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt1-V5

Plasmid

#35924

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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POLN_pDEST17

Plasmid

#64885

Purpose

bacterial expression of POLN for purification

Depositor

Richard Wood

Article

Takata et al Nucleic Acids Res. 2010 Jun;38(10):3233-

Insert

POLN (POLN Human)

Tags

Flag and His

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only

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