We narrowed to 246,895 results for: addgene

1. SLC5A3_pCSdest

   Plasmid

   #53836

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   SLC5A3 (SLC5A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pX330-sgRNA_Dgcr8_1

   Plasmid

   #73525

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

3. TAL2431

   Plasmid

   #36830

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   HDAC2-TALEN_Right (Hdac2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. miR-vector-RFP pCDNA

   Plasmid

   #97143

   Purpose

   for expression of miRNA and TagRFP

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Type

   Empty backbone

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MAC_C_SFXN1

   Plasmid

   #111676

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   SFXN1 (SFXN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. sgRNA3_GAL4UAS-Luciferase reporter

   Plasmid

   #64159

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBabe-puro-mIR-31

   Plasmid

   #26088

   Depositor

   Bob Weinberg

   Article

   Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

   Insert

   miR-31 (MIR31 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pWPXL-NP1

   Plasmid

   #72654

   Purpose

   Constitutive expression of NP1

   Depositor

   Ramon Trullas

   Article

   Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

   Insert

   NP1 (Nptx1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TAL2302

   Plasmid

   #36731

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   BRCA2-TALEN_Left (Brca2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

10. TAL2303

    Plasmid

    #36732

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA2-TALEN_Right (Brca2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. CEBPalpha NGFR

    Plasmid

    #44627

    Depositor

    Ellen Rothenberg

    Article

    Del Real et al Development. 2013 Mar;140(6):1207-19. do

    Insert

    CEBPalpha (Cebpa Mouse)
    

    Use

    Retroviral

    Tags

    IRES and truncated NGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pBABE-Src-Dasatinib-resistant

    Plasmid

    #26980

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    c-Src (SRC Chicken)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    T338I

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GalT-moxDendra2

    Plasmid

    #89789

    Purpose

    mammalian expression of GC localized moxDendra2

    Depositor

    Erik Snapp

    Article

    Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

    Insert

    GalT signal anchor transmembrane domain (B4GALT1 Human)
    

    Use

    Tags

    moxDendra2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Gfi1 NGFR

    Plasmid

    #44630

    Depositor

    Ellen Rothenberg

    Article

    Del Real et al Development. 2013 Mar;140(6):1207-19. do

    Insert

    Gfi1 (Gfi1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES and truncated NGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pFYF1327 EGFP Site#2

    Plasmid

    #47512

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG25-L-IFP-F[1] (clon 3)

    Plasmid

    #52899

    Purpose

    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[1]
    

    Use

    Vector for homologous recombination

    Tags

    Expression

    Mutation

    Promoter

    TEF promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pAG32-L-IFP-F[2] (clon 3)

    Plasmid

    #52900

    Purpose

    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[2]
    

    Use

    Vector for homologous recombination

    Tags

    Expression

    Mutation

    Promoter

    TEF promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pIS1 DICER1 long UTR

    Plasmid

    #21649

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    Dicer 1 long UTR (DICER1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. px330-USP13

    Plasmid

    #61812

    Purpose

    to make USP13 Knockout cell line

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    USP13 (USP13 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pET22b-BirA-CPDSalI

    Plasmid

    #38258

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    birA (birA E. coli)
    

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pGEX4T1-BirA

    Promoter

    T7

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only