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pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

Plasmid

#159914

Purpose

Mutagenesis of Rosa26 with SauCas9

Depositor

Larry Zweifel

Insert

Rosa26 gRNA (Gt(ROSA)26Sor Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-MetGFP

Plasmid

#37560

Depositor

David Rimm

Insert

MET (MET Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-MetGFP

Plasmid

#37560

Depositor

David Rimm

Insert

MET (MET Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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mu-FLT3Lv1-pTRIP

Plasmid

#163519

Purpose

Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

Depositor

Thomas Weber

Insert

murine-FLT3L variant 1 (Flt3l Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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mu-FLT3Lv1-pTRIP

Plasmid

#163519

Purpose

Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

Depositor

Thomas Weber

Insert

murine-FLT3L variant 1 (Flt3l Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-5' NUP155

Plasmid

#236644

Purpose

5' AAVLINK plasmid for NUP155 expression

Depositor

Zhonghua Lu

Insert

NUP155 (NUP155 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-5' NUP155

Plasmid

#236644

Purpose

5' AAVLINK plasmid for NUP155 expression

Depositor

Zhonghua Lu

Insert

NUP155 (NUP155 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-ON-shCtrl (neo)

Plasmid

#192076

Purpose

Lentivirus for Dox-inducible expression of a non-targeting control shRNA

Depositor

Roland Friedel

Insert

non-targeting randomized sequence

Use

Lentiviral

Promoter

H1 / Tet-responsive element

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-ON-shCtrl (neo)

Plasmid

#192076

Purpose

Lentivirus for Dox-inducible expression of a non-targeting control shRNA

Depositor

Roland Friedel

Insert

non-targeting randomized sequence

Use

Lentiviral

Promoter

H1 / Tet-responsive element

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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TRMT10C (RG9MTD1A-c113)

Plasmid

#110248

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

RG9MTD1 (TRMT10C Human)

Tags

TEV-His6-Flag

Expression

Bacterial

Promoter

T7 - lacO

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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TRMT10C (RG9MTD1A-c113)

Plasmid

#110248

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

RG9MTD1 (TRMT10C Human)

Tags

TEV-His6-Flag

Expression

Bacterial

Promoter

T7 - lacO

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABEmax-SpRY

Plasmid

#195279

Purpose

Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

Depositor

Carsten Lederer, Petros Patsali

Insert

human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABEmax-SpRY

Plasmid

#195279

Purpose

Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

Depositor

Carsten Lederer, Petros Patsali

Insert

human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-HA-CHAC1

Plasmid

#242710

Purpose

human CHAC1 gene expression

Depositor

Xingguo Zhu

Insert

CHAC1 (CHAC1 Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Multiple wobble mutations

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-HA-CHAC1

Plasmid

#242710

Purpose

human CHAC1 gene expression

Depositor

Xingguo Zhu

Insert

CHAC1 (CHAC1 Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Multiple wobble mutations

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pOK12

Plasmid

#49789

Purpose

E. coli cloning vector (KanR, low copy, blue/white selection)

Depositor

Joachim Messing

Article

Vieira et al Gene. 1991 Apr;100:189-94.

Type

Empty backbone

Use

Cloning vector

Promoter

lacZ

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pOK12

Plasmid

#49789

Purpose

E. coli cloning vector (KanR, low copy, blue/white selection)

Depositor

Joachim Messing

Article

Vieira et al Gene. 1991 Apr;100:189-94.

Type

Empty backbone

Use

Cloning vector

Promoter

lacZ

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-FLEX-SaCas9-U6-sgTh

Plasmid

#159901

Purpose

Mutagenesis of Th

Depositor

Larry Zweifel

Insert

Th (Th Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgTh

Plasmid

#159901

Purpose

Mutagenesis of Th

Depositor

Larry Zweifel

Insert

Th (Th Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEXfrt-SaCas9-U6-sgRosa26

Plasmid

#159915

Purpose

Mutagenesis of Rosa26

Depositor

Larry Zweifel

Insert

Rosa26 (Gt(ROSA)26Sor Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge