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pcDNA5-FRT-TetO-U2B''-fSNAPc

Plasmid

#106988

Purpose

open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Article

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

UB2" (SNRPB2 Human)

Tags

fSNAP

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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PBMBP-eGFP

Plasmid

#40976

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

MBP promoter fragment (Mbp Mouse)

Expression

Mammalian

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-Theo-3c-EGFP

Plasmid

#217526

Purpose

Theo-3c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

Depositor

Keisuke Fukunaga

Article

Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

Insert

Theo-3c riboswitch-regulated EGFP

Use

In vitro transcription-translation

Promoter

T7

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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p210 pCMV-CREM

Plasmid

#8395

Purpose

Cre with chimeric/β-globin intron within the Cre coding sequence

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

CMV

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pVW293

Plasmid

#139490

Purpose

Insertion of the pHSP12 promoter and 24 PP7 stem loops in the GLT1 locus using HIS marker in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pHSP12 24xPP7sl

Expression

Yeast

Mutation

WT

Promoter

pHSP12

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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C1-iRFP-iLid-CaaX

Plasmid

#155229

Purpose

membrane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

iRFP C-term tagged with the ilID motif and the CaaX sequence derived from Kras4b. Membrane control

Tags

iRFP-iLid

Expression

Mammalian

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Promoter

pBAD

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mCit-IRES-Neo

Plasmid

#155230

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mCitrine sensor

Use

Lentiviral

Tags

mCitrine

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pBad-Int Set Flipper

Plasmid

#38209

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Insert

Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Tags

His

Promoter

pBAD/AraC

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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mCherry-KRasCT

Plasmid

#99017

Purpose

Membrane marker

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

mCherry KRas CT

Expression

Mammalian

Promoter

CMV

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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PECAM-eGFP

Plasmid

#14688

Depositor

Victoria Bautch

Article

Kearney et al Blood. 2004 Jun 15. 103(12):4527-35.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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MAC3_C_VPS35L

Plasmid

#209698

Purpose

Expresses MAC3-tagged VPS35L in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Tags

MAC3-tag

Expression

Mammalian

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Foxa2-IRES-GFP

Plasmid

#33008

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A2 (Foxa2 Mouse)

Use

Retroviral

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pcNB1

Plasmid

#89241

Purpose

Resurfaced Cell-penetrating GFP nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB1 (GFP)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pX330S-6

Plasmid

#58782

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMEXNH3

Plasmid

#110079

Purpose

Analogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

His10 Tag - 3C cleavage site

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A-1x4

Plasmid

#58768

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pHACK-GAL4>QF2(newHA1)

Plasmid

#104873

Purpose

HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

T2A-QF2

Use

CRISPR

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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TRPML2-YFP

Plasmid

#18830

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML2 (Mcoln2 Mouse)

Tags

YFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX TIF1gamma

Plasmid

#15734

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma (TRIM33 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only