We narrowed to 9,297 results for: yeast expression

1. RS314-U6(PolII-Lsm)

   Plasmid

   #200772

   Purpose

   Pol II expression of U6 Lsm

   Depositor

   Aaron Hoskins

   Article

   Lipinski et al RNA. 2022 Dec;28(12):1606-1620. doi: 10.

   Insert

   Gal-SNR6-II
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc

   Plasmid

   #199760

   Purpose

   Elongation reporter construct to quantify elongation duration of 6_CGA stalling

   Depositor

   Brian Zid

   Article

   Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

   Insert

   synYFP[TTG/AGA]-6xArg[CGA]-nLuc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TetO7

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc

   Plasmid

   #199761

   Purpose

   Elongation reporter construct to quantify elongation duration of 6_AGA stalling

   Depositor

   Brian Zid

   Article

   Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

   Insert

   synYFP[TTG/AGA]-6xArg[AGA]-cLuc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TetO7

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. C-SWAT IAA17

   Plasmid

   #193319

   Purpose

   C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   IAA17
   

   Use

   Tags

   IAA17

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. C-SWAT Compatible pAIDA2(6FLAG)

   Plasmid

   #193321

   Purpose

   C-SWAT compatible vector for C-terminal tagging with TAP-AID*-6FLAG. osTIR1 is carried downstream of the C-terminal tag.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   TAP-AID*-6FLAG
   

   Use

   Tags

   TAP-AID*-6FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. C-SWAT AID*-6MYC osTIR1

   Plasmid

   #193320

   Purpose

   C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   AID*
   

   Use

   Tags

   6MYC

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C-SWAT AID*-6FLAG osTIR1

   Plasmid

   #182515

   Purpose

   C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   AID*
   

   Use

   Tags

   6FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C-SWAT IAA17-6FLAG

   Plasmid

   #182516

   Purpose

   C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   IAA17
   

   Use

   Tags

   6FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C-SWAT IAA17-3HA

   Plasmid

   #182517

   Purpose

   C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   IAA17
   

   Use

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C-SWAT IAA17-3MYC

    Plasmid

    #182518

    Purpose

    C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    IAA17
    

    Use

    Tags

    3MYC

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. C-SWAT IAA17-6FLAG

    Plasmid

    #182522

    Purpose

    C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 with CAN1 homology arms for genome integration.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    IAA17
    

    Use

    Tags

    6FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYTK001_E2At(2nd)

    Plasmid

    #183085

    Purpose

    2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    E2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYTK001_O2At(2nd)

    Plasmid

    #183086

    Purpose

    2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assembly

    Depositor

    Zhen Wang

    Article

    Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

    Insert

    O2A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMM0626

    Plasmid

    #129001

    Purpose

    pRPL18B-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConL2-pRPL18B-mRUBY2-tADH1-ConRE AmpR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMM0281

    Plasmid

    #128972

    Purpose

    Encodes VP16-CIB1 under pADH1 promoter

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    pscADH1-SV40NLS-VP16-CIB1-tscADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMM0520

    Plasmid

    #128983

    Purpose

    Overexpression plasmid for VP16-CIB1

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    SV40NLS-VP16-CIB1 LEU2 2_
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMM0627

    Plasmid

    #129002

    Purpose

    pTDH3-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConL2-pTDH3-mRUBY2-tADH1-ConRE AmpR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMM0494

    Plasmid

    #128981

    Purpose

    LEU2 preassembled entry vector

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    LEU2 5' homology-ConLS-sfGFP dropout-ConR1-LEU2 3' homology
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMM0625

    Plasmid

    #129000

    Purpose

    pREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    ConL2-pREV1-mRUBY2-tADH1-ConRE AmpR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMM0290

    Plasmid

    #128978

    Purpose

    Reporter plasmid, pZF(3BSopp)-yEVENUS

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    pZF(3BSop)-yEVENUS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge