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Plasmid#65779PurposeThe Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNADepositorInsertSaCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
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phFluc-WT
Plasmid#177714PurposeCytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019DepositorInserthFluc WT
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-STAT2
Plasmid#178449Purposedoxycycline-inducible expression of Human STAT2 in mammalian cellsDepositorInsertSTAT2 (STAT2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTH727-CEN-RLuc/staCFLuc
Plasmid#38211DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3 (=GPD)Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-EZH1_3
Plasmid#36361DepositorInsertEZH1 (EZH1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf106
Plasmid#12794PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf106
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHS1068
Plasmid#205962PurposeNZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promotersDepositorInsertDinG HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a_NEUROG1_P2A_Hygro_Barcode
Plasmid#120467PurposeBarcoded lentiviral vector to express NEUROG1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertNEUROG1 (NEUROG1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Txk-SH2
Plasmid#46526DepositorInsertTxk (Txk Mouse)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 138-258PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSMP-SUZ12_2
Plasmid#36391DepositorInsertSUZ12 (SUZ12 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Flag-hsDicer (Y971A/Y972A)
Plasmid#41590DepositorInserthsDicer (DICER1 Human)
UseTagsFlagExpressionMammalianMutationY971A/Y972APromoterCMVAvailable sinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTHMT_hnRNPF_deltaPLD
Plasmid#139115Purposeexpress FL hnRNPF without the prion like domain with N-terminal maltose binding protein tagDepositorInserthnRNPF (HNRNPF Human)
UseTagsExpressionBacterialMutationdelta365-415PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf37
Plasmid#12725PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf37
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf48
Plasmid#12736PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf48
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTET2-lucCP
Plasmid#198353PurposePart of Renilla reporter system, encodes Firefly luciferaseDepositorInsertFirefly Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterpTET2Available sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX Yes-SH2
Plasmid#46532DepositorInsertYes (YES1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 152-261PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-GMRWhite
Plasmid#165907PurposeBlasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pY005 (pcDNA3.1-hLb3Cpf1)
Plasmid#69977PurposeExpresses humanized Lb3Cpf1DepositorInserthLb3Cpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Sh3bp2-SH2
Plasmid#46484DepositorInsertSh3bp2 (SH3BP2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 450-561PromoterTacAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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EF1a_POU1F1_P2A_Hygro_Barcode
Plasmid#120473PurposeBarcoded lentiviral vector to express POU1F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertPOU1F1 (POU1F1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgGAL4-4
Plasmid#46916PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)DepositorInsertssgGAL4-4
Puromycin resistance and mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf40
Plasmid#12728PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf40
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-SP110
Plasmid#178448Purposedoxycycline-inducible expression of Human SP110 in mammalian cellsDepositorInsertSP110 (SP110 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
promoter J23110 (upstream of RBS, mRFP1)
Plasmid#117852PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertmedium-high promoter, RBS, mRFP1
UseSynthetic Biology; Escherichia coliTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
p53-(1-393)-R175H-N268D
Plasmid#35086DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains aa 1-393 - R175H-N268DPromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]
Plasmid#40806DepositorInsertMAP2K2 (MAP2K2 Human)
UseTagsHAExpressionMammalianMutationdelta(48-55) + S222D + S226DPromoterMSV-LTRAvailable sinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
Plasmid#172504PurposeEncodes the GwCas7x3 protein only for bacterial recombinant protein expression for purificationDepositorInsertGwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only