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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,532 results for: SUB

Showing: 6601 - 6650 of 9532 results

  2. pcDNA3+ hSlo2.2

    Plasmid
    #16214
    Depositor
    Larry Salkoff
    Insert
    KCNT1 potassium channel, subfamily (KCNT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    This construct lacks the first 53aa when compared…
    Promoter
    Available since
    Jan. 18, 2008
    Availability
    Academic Institutions and Nonprofits only
    16214-plasmid-map-sequence-id-248256

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  3. BK beta2 EGFP

    Plasmid
    #113558
    Purpose
    human BK beta2 subunit in C-term translational frame with EGFP (EGFP-N1)
    Depositor
    Robert Brenner
    Insert
    Kcnmb2 (KCNMB2 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113558-plasmid-map-sequence-id-218921

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  4. KCNQ4-Myc

    Plasmid
    #197329
    Purpose
    Plasmid expressing an antigen targeting the KCNQ4/Kv7/4 K+ channel subunit
    Depositor
    Thomas Jespersen
    Insert
    KCNQ4 (KCNQ4 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197329-plasmid-map-sequence-id-406745

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  5. Myr-(iSH2-p85)-p110alpha-Myc

    Plasmid
    #1410
    Depositor
    Lewis Cantley
    Insert
    PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    SRalpha promoter
    Available since
    June 16, 2005
    Availability
    Academic Institutions and Nonprofits only
    1410-plasmid-map-sequence-id-352163

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  6. BK beta4 EGFP

    Plasmid
    #113560
    Purpose
    human BK beta4 subunit in C-term translational frame with EGFP (EGFP-N1)
    Depositor
    Robert Brenner
    Insert
    Kcnmb4 (KCNMB4 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113560-plasmid-map-sequence-id-218925

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  7. BK beta1 EGFP

    Plasmid
    #113557
    Purpose
    mouse BK beta1 subunit in C-terminal translational frame with EGFP
    Depositor
    Robert Brenner
    Insert
    Kcnmb1 (Kcnmb1 Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113557-plasmid-map-sequence-id-218875

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  8. p202 Dync1li1 wt

    Plasmid
    #26450
    Depositor
    Elizabeth Fisher
    Insert
    cytoplasmic dynein light intermediate chain 1 (Dync1li1 Mouse)
    Use
    Subcloning and sequencing
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Nov. 8, 2010
    Availability
    Academic Institutions and Nonprofits only

  9. pB2H2*

    Plasmid
    #163834
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and TCPTP(R222M)
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    TCPTP R222M
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163834-plasmid-map-sequence-id-337601

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  10. PHB1

    Plasmid
    #212153
    Purpose
    For PHB production in E. coli (PT7-pMB1).
    Depositor
    Kang Zhou
    Insert
    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212153-plasmid-map-sequence-id-420764

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  11. BK beta2 ECFP

    Plasmid
    #113562
    Purpose
    human BK beta2 subunit in C-term translational frame with ECFP (ECFP-N1)
    Depositor
    Robert Brenner
    Insert
    Kcnmb2 (KCNMB2 Human)
    Use
    Tags
    ECFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113562-plasmid-map-sequence-id-218927

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  12. pOX + hSlo2.2

    Plasmid
    #16215
    Depositor
    Larry Salkoff
    Insert
    KCNT1 potassium channel, subfamily (KCNT1 Human)
    Use
    Xenopus oocyte expression
    Tags
    Expression
    Mutation
    This construct lacks the first 53aa when compared…
    Promoter
    Available since
    Jan. 18, 2008
    Availability
    Academic Institutions and Nonprofits only

  13. pIND4-GFP-MmXYRS

    Plasmid
    #222745
    Purpose
    Expresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinker
    Depositor
    Steven Boxer
    Inserts
    sfGFP
    MmXYRS
    Mm Pyl tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    Contains the following substitutions: V346A, W348…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222745-plasmid-map-sequence-id-442565

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  14. mBeta4/pCNA3.1

    Plasmid
    #197316
    Purpose
    Plasmid expressing an antigen used to generate an antibody that targets the BK beta4 potassium channel subunit
    Depositor
    Robert Brenner
    Insert
    Slo B4 (Kcnmb4 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    197316-plasmid-map-sequence-id-388157

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  15. Kv1.1/RBG4

    Plasmid
    #196943
    Purpose
    Plasmid expressing an antigen targeting the Kv1.1 potassium channel subunit
    Depositor
    James Trimmer
    Insert
    Kv1.1 (Kcna1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196943-plasmid-map-sequence-id-388530

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  16. KChIP2/RBG4; 9QM3/RBG4

    Plasmid
    #196944
    Purpose
    Plasmid expressing an antigen targeting the KChIP2b potassium channel subunit
    Depositor
    James Trimmer
    Insert
    KChIP2 (Kcnip2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196944-plasmid-map-sequence-id-391763

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  17. GABAAR alpha5 HA/pCI

    Plasmid
    #197322
    Purpose
    Plasmid expressing an antigen targeting the GABA-A receptor alpha5 subunit
    Depositor
    Stephen Moss
    Insert
    GABA A alpha 5 (GABRA5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    197322-plasmid-map-sequence-id-392915

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  18. pB2H12*

    Plasmid
    #163838
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12 (Y64A)
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    PTPN12 Y64A
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163838-plasmid-map-sequence-id-337609

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  19. pB2H12

    Plasmid
    #163837
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    PTPN12 E57D
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163837-plasmid-map-sequence-id-337607

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  20. pB2H6*

    Plasmid
    #163836
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1 (R459M)
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    SHP1 R459M
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163836-plasmid-map-sequence-id-337604

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  21. pB2Hopt*

    Plasmid
    #163831
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B(C215S)
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    PTP1B C215S
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163831-plasmid-map-sequence-id-337592

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  22. L-SACSIN-74

    Plasmid
    #166526
    Purpose
    scFv of a human scaffold targeting Sacsin, DnaJ homolog subfamily C member 29. Antigen coverage aa 84-339 of 4579
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166526-plasmid-map-sequence-id-329139

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  23. BK beta4 ECFP

    Plasmid
    #113564
    Purpose
    human BK beta4 subunit in C-term translational frame with ECFP (ECFP-N1)
    Depositor
    Robert Brenner
    Insert
    Kcnmb4 (KCNMB4 Human)
    Use
    Tags
    ECFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113564-plasmid-map-sequence-id-218936

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  24. pJK439

    Plasmid
    #72478
    Purpose
    Produces Thermoplasma acidophilum citrate synthase pseudo-dimer
    Depositor
    T. J. Kappock
    Insert
    citrate synthase (artificial dimer)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted NSTCLVPRGSPV sequence between C- and N-t…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72478-plasmid-map-sequence-id-134617

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  25. pJK573

    Plasmid
    #72456
    Purpose
    Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~57 silent substitutions
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72456-plasmid-map-sequence-id-133910

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  26. pJK569

    Plasmid
    #72455
    Purpose
    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    synthetic gene with ~51 silent substitutions
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72455-plasmid-map-sequence-id-133906

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  27. cacna2d1 pcDNA3

    Plasmid
    #206103
    Purpose
    expression of rat alpha2delta-1 calcium channel auxillary subunit
    Depositor
    Annette Dolphin
    Insert
    cacna2d1 (Cacna2d1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206103-plasmid-map-sequence-id-415385

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  28. Kv4.3 long/RBG4

    Plasmid
    #196946
    Purpose
    Plasmid expressing an antigen targeting the Kv4.3 potassium channel subunit
    Depositor
    James Trimmer
    Insert
    Kv4.3 (Kcnd3 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196946-plasmid-map-sequence-id-388533

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  29. GluR2 FL/pRK5

    Plasmid
    #196948
    Purpose
    Plasmid expressing an antigen targeting the GluR2/GluA2 AMPA receptor subunit
    Depositor
    James Trimmer
    Insert
    GluR2 (FL) (Gria2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196948-plasmid-map-sequence-id-387880

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  31. BK beta3 EGFP

    Plasmid
    #113559
    Purpose
    human BK beta3 subunit in C-term translational frame with EGFP (EGFP-N1)
    Depositor
    Robert Brenner
    Insert
    Kcnmb3 (KCNMB3 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113559-plasmid-map-sequence-id-218923

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  32. hSlo2.1 Slick/pCDNA3

    Plasmid
    #197317
    Purpose
    Plasmid expressing an antigen used to generate an antibody that targets the Slo2.1 Slick K+ channel subunit
    Depositor
    Christopher Lingle
    Insert
    Slo2.1 (Kcnt2 Mouse)
    Use
    Tags
    7xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197317-plasmid-map-sequence-id-388158

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  33. pX459-puro-hPKAalpha

    Plasmid
    #185379
    Purpose
    For mammalian expression of guide RNA: caccgTTTGAACGAATCAAGACCCT that targets human PKA subunit alpha
    Depositor
    Ayaz Najafov
    Insert
    PRKACA (PRKACA Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185379-plasmid-map-sequence-id-363450

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  34. cacna2d1 pCAGGS

    Plasmid
    #206104
    Purpose
    expression of rat alpha2delta-1 calcium channel auxillary subunit
    Depositor
    Annette Dolphin
    Insert
    cacna2d1 (Cacna2d1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/B-actin
    Available since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    206104-plasmid-map-sequence-id-413011

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  35. Z-FARSB-5

    Plasmid
    #166556
    Purpose
    scFv of a human scaffold targeting phenylalanyl-tRNA synthetase, beta subunit. Antigen coverage aa 1-589 of 589, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166556-plasmid-map-sequence-id-329264

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  38. SF3A1

    Plasmid
    #25608
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SF3A1 (SF3A1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Ubiquitin-like domain
    Promoter
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. pCRII-Topo Couptf1

    Plasmid
    #45870
    Depositor
    Jeffrey Macklis
    Insert
    Couptf1 in situ probe (Nr2f1 Mouse)
    Use
    In situ
    Tags
    Expression
    Mutation
    contains bp# 1755-2381 of NM_010151.2
    Promoter
    Available since
    July 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. RAC3

    Plasmid
    #38821
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RAC3 (rac3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  41. pET29b(+)_gp1(A11V)

    Plasmid
    #225131
    Purpose
    Phage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)
    Depositor
    Matej Butala
    Insert
    gp1 (A11V)
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Alanine 11 to Valine
    Promoter
    T7 promoter
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225131-plasmid-map-sequence-id-448443

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  42. pIND4-RC-M210TAG-A7

    Plasmid
    #223015
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for 3-nitrotyrosine
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    A7 RS
    pylT tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    223015-plasmid-map-sequence-id-444740

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  43. cacnb1b pCAGGS

    Plasmid
    #206102
    Purpose
    expression of rat beta1b calcium channel auxillary subunit
    Depositor
    Annette Dolphin
    Insert
    cacnb1b (Cacnb1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/B-actin
    Available since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    206102-plasmid-map-sequence-id-413014

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  44. Kv1.4/RBG4

    Plasmid
    #196938
    Purpose
    Plasmid expressing an antigen targeting the Kv1.4 potassium channel subunit
    Depositor
    James Trimmer
    Insert
    Kv1.4 (Kcna4 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    196938-plasmid-map-sequence-id-387886

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  45. cacnb1b pcDNA3

    Plasmid
    #206105
    Purpose
    expression of rat beta1b calcium channel auxillary subunit
    Depositor
    Annette Dolphin
    Insert
    cacnb1b (Cacnb1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206105-plasmid-map-sequence-id-406744

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  46. GluR1-Flop/pRK5

    Plasmid
    #197308
    Purpose
    Plasmid expressing an antigen targeting the GluR1/GluA1 AMPA receptor subunit
    Depositor
    Robert Wenthold
    Insert
    GluR1-Flop (Gria1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197308-plasmid-map-sequence-id-388144

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  47. Kv1.4/GW1

    Plasmid
    #197331
    Purpose
    Plasmid expressing an antigen targeting the Kv1.4 potassium channel subunit
    Depositor
    Morgan Sheng
    Insert
    Kv1.4 (Kcna4 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    197331-plasmid-map-sequence-id-388230

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  48. RCav3.1/pcDNA3-HE3

    Plasmid
    #197324
    Purpose
    Plasmid expressing an antigen targeting the Cav3.1 calcium channel subunit
    Depositor
    Edward Perez-Reyes
    Insert
    Cav3.1 (Cacna1g Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197324-plasmid-map-sequence-id-388203

    Enlarge

Showing: 6601 - 6650 of 9532 results