We narrowed to 9,603 results for: FIE

1. Human NANOS3-P2A-mCherry knock-in targeting construct

   Plasmid

   #59721

   Purpose

   Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

   Depositor

   Jacob Hanna

   Article

   Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

   Insert

   P2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. RHBDD1 pmRFP-N2 (1390)

   Plasmid

   #62049

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   RHBDD1 (RHBDD1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pDONR223-MCU

   Plasmid

   #31726

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Use

   Pdonr gateway

   Tags

   None

   Expression

   Mutation

   *

   Promoter

   None

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTet-O-NEUROG1-T2A-PuroR

   Plasmid

   #162339

   Purpose

   Lentiviral expression of NEUROG1 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   NEUROG1 (NEUROG1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

   Plasmid

   #78304

   Purpose

   Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-nlsLexA::GADfluw-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

   Plasmid

   #129530

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIC3Nme
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Expression

   Mutation

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. ETV2_pet28a

   Plasmid

   #131665

   Purpose

   Bacterial expression of ETS gene ETV2

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETV2 (ETV2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM

   Plasmid

   #183150

   Purpose

   Expression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)

   Depositor

   Assaf Mosquna

   Article

   Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

   Inserts

   Lex-ER-B112
   Zif268 DBD - hPRLBD - MSN2AD
   

   Use

   For yeast genomic integration

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-FLEX-ExSYTE

   Plasmid

   #216263

   Purpose

   Cre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

   Depositor

   Susumu Tomita

   Article

   Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

   Insert

   ExSYTE P2A EGFP - Rat DTE
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

    Plasmid

    #115789

    Purpose

    Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    NDP (NDP Human)
    

    Use

    Tags

    alkaline phosphatase and flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-7aa-ActA-pEGFP-C1

    Plasmid

    #177406

    Purpose

    To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

    Depositor

    David Andrews

    Article

    Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

    Insert

    ActA tail anchor (actA Listeria monocytogenes)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. FEV_pet28a

    Plasmid

    #131668

    Purpose

    Bacterial expression of ETS gene FEV

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    FEV (FEV Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TIMP1-bio-His

    Plasmid

    #52089

    Purpose

    Expresses full-length Metalloproteinase inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TIMP1 (TIMP1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-uTEV1-NES

    Plasmid

    #171006

    Purpose

    HiLITR protease with C-terminal NNES (Cytoplasmic)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-NES
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. ELF4_pet28a

    Plasmid

    #131661

    Purpose

    Bacterial expression of ETS gene ELF4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF4 (ELF4 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330_ATP1A1_G7_Dual_sgRNA

    Plasmid

    #173206

    Purpose

    Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD02_CBASS_Escherichia_coli_CVM_N37067PS

    Plasmid

    #224403

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli CVM N37067PS

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    EcCdnF_AGS-C_SLATT6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD02_CBASS_Escherichia_coli_BIDMC103

    Plasmid

    #224402

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli BIDMC103

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    EcCdnF_AGS-C_SLATT6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD02_CBASS_Escherichia_coli_K71-77

    Plasmid

    #224398

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli K71-77

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    Phospholipase_EcCdnE_DisA-like
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD02_CBASS_Escherichia_coli_RU1_BHI6

    Plasmid

    #224397

    Purpose

    For expression of the type I CBASS system encoded by Escherichia coli RU1 BHI6

    Depositor

    Philip Kranzusch

    Article

    Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

    Insert

    Cap15_EcCdnE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge