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pAN-PBAD-sgRNA-A2NT

Plasmid

#62257

Purpose

expression of A2NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A2NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pXWS20tetO

Plasmid

#160826

Purpose

20 repeats of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(20 X tetO)-t

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJH4.67

Plasmid

#26948

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

gamma-tubulin (tbg-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pAN-OR

Plasmid

#62307

Purpose

synthetic circuit: OR gate

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
A4NT sgRNA
A2NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PA2, PPhlF, and pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pERB219

Plasmid

#61501

Purpose

Const. Halotag-GFP-PACT + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-PACT
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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BiDi-[mEGFP]mCherry2

Plasmid

#182875

Purpose

Bi-directional expression vector of monomers

Depositor

Salvatore Chiantia

Article

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

mCherry2 and mEGFP

Expression

Mammalian

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCri-13a

Plasmid

#61321

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and MISTIC-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pDsRed-miR9 Sensor

Plasmid

#22742

Depositor

Lynn Hudson

Article

Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

Insert

microRNA 9 binding sites

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pI1-MTU-DO-LEU

Plasmid

#160013

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Leucine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:lyn-tagRFPt

Plasmid

#192354

Purpose

lyn-tagRFPt expression under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

lyn-tagRFPT

Tags

tagRFPT

Expression

Mammalian

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAB164

Plasmid

#101667

Purpose

Opto-T7RNAP*(563), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), half expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-SNAP-Smo

Plasmid

#31617

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Zebrafish, avian expression

Tags

SNAP

Expression

Mammalian and Worm

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pICSL50008

Plasmid

#50314

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, GFP (A. victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-BoxB-A114-N40

Plasmid

#50562

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-BoxB-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAN-PA2-RFP

Plasmid

#62248

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Expression

Bacterial

Promoter

pA2

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-CLIPf-WPRE

Plasmid

#104107

Purpose

Expresses CLIPf under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

CLIPf

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAct1IsGFP-1

Plasmid

#38775

Depositor

Peggy Lemaux

Article

Cho et al Plant Cell Rep. 2000 Nov;19(11):1084-108

Insert

Actin promoter and intron

Use

Plant transformation

Tags

nos 3' terminator and sGFP (S65T)

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pJMP12068

Plasmid

#222356

Purpose

Tn7 integrative vector with PabstBR promoter expression cassette and kanR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFF706

Plasmid

#61448

Purpose

Light-inducible SCRIBE_kanR(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_kanR(ON)

Use

Synthetic Biology

Promoter

pDawn

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pXWS10tetO

Plasmid

#160825

Purpose

10 repeats of tetO sponge

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(10 X tetO)-t

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge