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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,772 results for: PGK

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Showing: 2641 - 2660 of 2772 results

  1. pIEforRed

    Plasmid
    #185892
    Purpose
    In vivo gene amplification (HapAmp) of EforRed expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Publication
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>EforRed>TPGK1-PBTS1> RPL25(partial; Arm2)
    Expression
    Yeast
    Mutation
    None
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185892-plasmid-map-sequence-id-370865

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  5. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid
    #191529
    Purpose
    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2BK_mNG_hSLBP(18-108)
    Use
    CRISPR and Synthetic Biology
    Tags
    mNeonGreen (mNG)
    Expression
    Mammalian
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191529-plasmid-map-sequence-id-378106

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  6. pIAeBlueHPV16LR

    Plasmid
    #185894
    Purpose
    In vivo gene amplification (HapAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Publication
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185894-plasmid-map-sequence-id-374392

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  7. cTRE-LSL-GFP-IRES-Cas9-sgCR8

    Plasmid
    #135668
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgCR8
    Use
    Mouse Targeting
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135668-plasmid-map-sequence-id-325838

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  8. pKLV-gRNA-CTBP2-MGMT_1

    Plasmid
    #136419
    Purpose
    Lentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.
    Depositor
    Massimo Squatrito
    Insert
    U6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    136419-plasmid-map-sequence-id-271761

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  11. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

    Plasmid
    #169657
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169657-plasmid-map-sequence-id-365632

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  12. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

    Plasmid
    #169659
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169659-plasmid-map-sequence-id-364557

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  15. pJF87

    Plasmid
    #253754
    Purpose
    Encodes GFP_AID* and pALD6-OsTIR1(F74G, S210A) expression cassettes. The OsTIR1(F74G, S210A) coding sequence is codon optimized for S. cerevisiae.
    Depositor
    Matthias Heinemann
    Inserts
    GFP_AID*
    OsTIR1
    Tags
    mAID*-tag
    Expression
    Yeast
    Mutation
    F74G, S210A
    Promoter
    ALD6 and TEF1
    Available since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    253754-plasmid-map-sequence-id-507832

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  17. VEGF120TAG

    Plasmid
    #200946
    Purpose
    Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation
    Depositor
    Elizabeth Kirby
    Publication
    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103
    Inserts
    VEGF120TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    Use
    Lentiviral
    Tags
    6x HIS
    Mutation
    amber stop codon 101 bp from ATG
    Promoter
    Ef1a and hPGK
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    200946-plasmid-map-sequence-id-396320

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  18. VEGF164TAG

    Plasmid
    #200947
    Purpose
    Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation
    Depositor
    Elizabeth Kirby
    Publication
    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103
    Inserts
    VEGF164TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    Use
    Lentiviral
    Tags
    6x HIS
    Mutation
    amber stop codon 101 bp from ATG
    Promoter
    Ef1a and hPGK
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    200947-plasmid-map-sequence-id-396321

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Showing: 2641 - 2660 of 2772 results