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pBbdCas9S_P0826L-sgRNAflaB

Plasmid

#149648

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0826L

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNArodA

Plasmid

#149655

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTS-sgRNAflaB

Plasmid

#149644

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_PresTL-sgRNAflaB

Plasmid

#149645

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, PresTL

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0026-sgRNAflaB

Plasmid

#149646

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0026

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0826S-sgRNAflaB

Plasmid

#149647

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0826S

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAftsI

Plasmid

#149649

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAftsI

Plasmid

#149650

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNAftsI

Plasmid

#149563

Purpose

ftsI gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAftsI

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2H_Psyn-sgRNAmreB

Plasmid

#149567

Purpose

mreB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1052B (Ubiq-Fluc_Ubiq-Rluc)

Plasmid

#132426

Purpose

Express Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Inserts

Ubiq promoter
firefly luciferase
T2A-eGFP
Renilla Luciferase-SV40-P10
reversed ubiq promoter

Expression

Insect

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pSAC16

Plasmid

#137965

Purpose

Expresses Zea mays FNR and Prochlorococcus marinus NATL2A SIR

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Inserts

Zea mays Ferredoxin-NADP reductase
Procholoroccus marinus NATL2A sulfite reductase

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSAC17

Plasmid

#137966

Purpose

Expresses Zea mays FNR and Prochlorococcus marinus MIT9211 SIR

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Inserts

Zea mays Ferredoxin-NADP reductase
Procholoroccus marinus MIT9211 sulfite reductase

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE56

Plasmid

#247034

Purpose

Glucocorticoid Receptor pathway reporter; lentiviral expression of NR3C1 (GR) DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NR3C1 (GR)

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDGB1a2+pNOS_Red-F_NOSt

Plasmid

#170887

Purpose

GoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Forward orientation

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

Red-F

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

p.S286Y Red mutant

Promoter

pNOS

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C9orf72

Plasmid

#83437

Purpose

Expresses GFP-C9orf72 in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

C9orf72 (C9orf72 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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FTL

Plasmid

#100750

Purpose

Part of FerriTag expression system. Untagged Ferritin light chain in mammalian expression vector.

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

FTL (FTL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GFP-FF3

Plasmid

#11662

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3 NSE promoter

Plasmid

#11606

Depositor

Rosalyn Adam

Article

Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

Insert

NSE promoter (ENO2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI3M

Plasmid

#249061

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI3M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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