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We narrowed to 31,675 results for: PLE

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Showing: 2661 - 2680 of 31675 results

  1. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

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  3. pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid
    #228558
    Purpose
    Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus
    Depositor
    Neville Sanjana
    Article
    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103
    Insert
    dhfCas13d (N2V8)
    Use
    Lentiviral
    Tags
    NLS-HA
    Mutation
    R239A/H244A/R858A/H863A
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    228558-plasmid-map-sequence-id-457180

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  4. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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  5. pLexM-HsPQLC1(Δ100-125/GGGS/Δ257-271)-TEV-GFP-His8

    Plasmid
    #250427
    Purpose
    Expression of C-terminally TEV-GFP-His8 Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a GGGS Linker; Δ257-271) in Mammalian Cells
    Depositor
    Simon Newstead
    Insert
    PQLC1 (SLC66A2 Human)
    Tags
    TEV-GFP-8xHis
    Expression
    Mammalian
    Mutation
    Δ100-125 (Replaced with GGGS), Δ257-271
    Promoter
    Chicken-beta-actin promoter/CMV Enhancer
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250427-plasmid-map-sequence-id-498990

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  6. pLexM-HsPQLC1(Δ100-125/3xGGGS/Δ257-271)-TEV-GFP-His8

    Plasmid
    #250429
    Purpose
    Expression of C-terminally TEV-GFP-His8 Tagged HsPQLC1 (Δ100-125 - with these residues replaced by 3xGGGS Linker; Δ257-271) in Mammalian Cells
    Depositor
    Simon Newstead
    Insert
    PQLC1 (SLC66A2 Human)
    Tags
    TEV-GFP-8xHis
    Expression
    Mammalian
    Mutation
    Δ100-125 (Replaced with 3xGGGS), Δ257-271
    Promoter
    Chicken-beta-actin promoter/CMV Enhancer
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250429-plasmid-map-sequence-id-498850

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  8. pTwist-SARS-CoV-2 Spike-deltaC18 SA501.V2 (Triple RBD) R683G (GGC)

    Plasmid
    #212387
    Purpose
    Encodes SARS-CoV-2 spike K417N,E484K,N501Y,R683G for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike K417N,E484K,N501Y,R683G (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    K417N,E484K,N501Y,R683G
    Promoter
    CAG
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212387-plasmid-map-sequence-id-431626

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  9. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

    Plasmid
    #189636
    Purpose
    Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries
    Depositor
    Manuel Kaulich
    Article
    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.
    Insert
    hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    189636-plasmid-map-sequence-id-414907

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  10. pKG03572_pLentiX1-(TRE-mRuby2-bGH)-cHS4.core-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #252557
    Purpose
    Inducible Tet all-in-one circuit with divergent syntax and intergenic cHS4 core insulator, lentiviral vector
    Depositor
    Kate Galloway
    Article
    Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.
    Insert
    rtTA-P2A-mGreenLantern
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EFS (human elongation factor 1-alpha - short/intr…
    Available Since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    252557-plasmid-map-sequence-id-503968

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  11. pKG03576_pLentiX1-(TRE-mRuby2-bGH)-cHS4.full-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #252560
    Purpose
    Inducible Tet all-in-one circuit with divergent syntax and intergenic cHS4 full insulator, lentiviral vector
    Depositor
    Kate Galloway
    Article
    Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.
    Insert
    rtTA-P2A-mGreenLantern
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EFS (human elongation factor 1-alpha - short/intr…
    Available Since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    252560-plasmid-map-sequence-id-503972

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  12. pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

    Plasmid
    #189635
    Purpose
    Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries
    Depositor
    Manuel Kaulich
    Article
    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.
    Insert
    h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    189635-plasmid-map-sequence-id-414905

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  13. pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP

    Plasmid
    #122955
    Purpose
    Lentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expression
    Depositor
    Pierre Vanderhaeghen
    Article
    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.
    Insert
    NOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
    Use
    Lentiviral
    Tags
    6xhis tag
    Mutation
    deletion of EGF repeats from full length NOTCH2NLB
    Promoter
    CAG promoter
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122955-plasmid-map-sequence-id-244415

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  20. pKG03571_pLentiX1-TRE-mRuby2-bGH-cHS4.core-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #252558
    Purpose
    Inducible Tet all-in-one circuit with downstream tandem syntax and intergenic cHS4 core insulator, lentiviral vector
    Depositor
    Kate Galloway
    Article
    Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.
    Insert
    rtTA-P2A-mGreenLantern
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EFS (human elongation factor 1-alpha - short/intr…
    Available Since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    252558-plasmid-map-sequence-id-503970

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Showing: 2661 - 2680 of 31675 results