We narrowed to 14,414 results for: UTY

1. pCDNAIIIB rac1 QL

   Plasmid

   #61633

   Purpose

   mammalian expression of rac1 constitutively active mutant

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q61L, constitutively active

   Promoter

   CMV

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

2. BCL9-BirA*

   Plasmid

   #109147

   Purpose

   To generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

   Depositor

   Mariann Bienz

   Article

   van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

   Insert

   BCL9 (BCL9 Human)
   

   Use

   Tags

   BirA(R118G)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEMS1725

   Plasmid

   #105870

   Purpose

   High copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   iCreERT2-FFrt-KanR-FFrt
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLCKO_PSMD1_sgRNA_1

   Plasmid

   #74180

   Purpose

   lentiviral vector expressing sgRNA targeting PSMD1

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   PSMD1 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 Promoter

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-FAM86A

   Plasmid

   #85117

   Purpose

   bacterial expression of FAM86A

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EEF2KMT (FAM86A Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FUG-T2A-Cas9

   Plasmid

   #75346

   Purpose

   Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP (via t2a)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

   Plasmid

   #124660

   Purpose

   reporter protein which emits bright blue fluorescence

   Depositor

   Yutaka Kuroda

   Article

   Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

   Insert

   Gaussia luciferase-C9D-TGinsert
   

   Use

   Tags

   IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

   Expression

   Bacterial

   Mutation

   changed glutamic acid 100 to alanine, and glycine…

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNCST-AausFP2

   Plasmid

   #129500

   Purpose

   Expresses AausFP2 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. tet-pLKO shMKP5.v2 puro

   Plasmid

   #87793

   Purpose

   Expresses an inducible short hairpin targeting human MKP5 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP5 (DUSP10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE tight

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. tet-pLKO shMKP5.v1 puro

    Plasmid

    #87792

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FKBP-Stx3-mCitrine

    Plasmid

    #92428

    Purpose

    Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx3 (Stx3 Rat)
    

    Use

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNCST-AausGFP

    Plasmid

    #129503

    Purpose

    Expresses AausGFP constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST47-CYFIP2.GFP

    Plasmid

    #109140

    Purpose

    GFP-tagged CYFIP2 expression construct

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP2 (CYFIP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FKBP-Stx4-mCitrine

    Plasmid

    #92427

    Purpose

    Expression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Use

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-mTDGa.1

    Plasmid

    #81051

    Purpose

    bacterial expression of catalytically dead murine TDG

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    asparagine 151 changed to alanine

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Hy_pMT Flag-Hel25E K91N

    Plasmid

    #110121

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    K91N mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Hy_pMT Flag-Hel25E D193E

    Plasmid

    #110120

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    D193E mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tet-pLKO shMKP1.v2 puro

    Plasmid

    #87791

    Purpose

    Expresses an inducible short hairpin targeting human MKP1 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP1 (DUSP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

    Plasmid

    #105037

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

    Plasmid

    #105038

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge