We narrowed to 3,462 results for: mpo

1. pMXS-IRES-Blast POLA1 ISCmut

   Plasmid

   #160808

   Purpose

   Express ISC mutant POLA1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLA1 ISCmut (POLA1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon optimized, C1354S, C1359S, C1377S, C1380S

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-Hyg-Snrnp40-skywarp-CRISPR-resistant

   Plasmid

   #134252

   Purpose

   Lentivector encoding CRISPR-resistant skywarp form of Snrnp40 (missing exon 5)

   Depositor

   Bruce Beutler

   Article

   Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

   Insert

   Snrp40 (Snrnp40 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   deleted amino acids 179-219, and mutated coding s…

   Promoter

   EF1a

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLuc-Optop38i-dsm

   Plasmid

   #89748

   Purpose

   Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   Optop38i dark-state mutant
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   C450A mutation of AsLov2Jalpha

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBK-CMV-mpx

   Plasmid

   #191826

   Purpose

   mpx probe generation for in situ

   Depositor

   Jason Berman

   Article

   Biggs et al JCI Insight. 2022 Dec 22;7(24):e150849.

   Insert

   mpx cDNA (mpx Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV2/9 REP-AAP-ΔCAP

   Plasmid

   #205991

   Purpose

   Rep and AAP protein expression for AAV CREATE libraries

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   AAV9 Rep and AAP proteins for CREATE libraries
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQCXIZ GFP PODXL delta PBM + Ezrin

   Plasmid

   #202418

   Purpose

   Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2) fused to EZRIN

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   Ezrin and GFP

   Expression

   Mutation

   delete PDZ binding motif

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-mCherry-ARHGEF2

   Plasmid

   #207959

   Purpose

   Expression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tag

   Depositor

   Johanna Ivaska

   Article

   Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

   Insert

   ARHGEF2 (ARHGEF2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169192

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Unspecified

   Tags

   6His-TEV

   Expression

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-pro-Il1b-C-Flag

   Plasmid

   #75131

   Purpose

   Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   interleukin 1 beta (Il1b Mouse)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   (please see depositor comments below)

   Promoter

   CMV

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON)(TRE-SEAP

    Plasmid

    #210513

    Purpose

    expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON and SEAP reporter in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Inserts

    secreted alkaline phosphatase
    CMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and TetO

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

    Plasmid

    #159697

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

    Plasmid

    #159696

    Purpose

    E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)
    

    Use

    Tags

    His6-Halotag-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

    Plasmid

    #159698

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-EF-FH-TAZ S89A-ires-blast

    Plasmid

    #52084

    Purpose

    Lentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tags

    Depositor

    Yutaka Hata

    Article

    Yang et al Mol Cell Biol. 2014 Feb 18.

    Insert

    TAZ S89A (WWTR1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    S89A

    Promoter

    EF1a

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169193

    Purpose

    To express SARS-CoV-2 Papain-like protease in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP

    Plasmid

    #216480

    Purpose

    lentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    TAZ4SA (WWTR1 Human)
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    S66A, S89A, S117A, S311A

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CMV TRE3G Neo GFP-Lamin A wildtype

    Plasmid

    #118709

    Purpose

    Lentiviral TET-ON inducible GFP-Lamin A wildtype

    Depositor

    Tom Misteli

    Article

    Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

    Insert

    GFP-Lamin A human wildtype (LMNA Human)
    

    Use

    Lentiviral; Tet-on inducible

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TRE3G (TET-ON)

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

    Plasmid

    #216479

    Purpose

    lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    wtYAP (YAP1) (YAP1 Human)
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-EF-FH-TAZ-ires-blast

    Plasmid

    #52083

    Purpose

    Lentiviral expression vector for TAZ with N-terminal FLAG and His tags

    Depositor

    Yutaka Hata

    Article

    Yang et al Mol Cell Biol. 2014 Feb 18.

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-N-Flag-NLRP3 711-1034

    Plasmid

    #75141

    Purpose

    Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 711-1034

    Promoter

    CMV

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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