We narrowed to 152,916 results for: Des

1. pORANGE Shank2-GFP KI

   Plasmid

   #131496

   Purpose

   Endogenous tagging of Shank2: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Shank2, Shank2 Rat, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

   Plasmid

   #113681

   Purpose

   A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   de-catalyzed SaCas9
   

   Use

   AAV and CRISPR

   Tags

   KRAB and NLS

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE Doc2A-GFP KI

   Plasmid

   #131478

   Purpose

   Endogenous tagging of Doc2a: C-terminal (amino acid position: L402)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Doc2a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP303-pAAV-CMV-dSaCas9-VP64-pA

   Plasmid

   #113678

   Purpose

   A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   de-catalyzed SaCas9
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   NLS and VP64

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-IP3KC

   Plasmid

   #134603

   Purpose

   Expresses IP3KC GFP tagged

   Depositor

   Christophe Erneux

   Article

   Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

   Insert

   IP3KC (ITPKC Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJET1.2-U3

   Plasmid

   #173156

   Purpose

   Single guide RNA cassette under U3 promoter

   Depositor

   Piotr Ziolkowski

   Article

   Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

   Insert

   sgRNA cassette under U3 promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   promoter region of the U3C snRNA gene (Marshallsa…

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXY349

   Plasmid

   #200262

   Purpose

   Expressing FtsW-TagRFP-T in bacterial cells

   Depositor

   Jie Xiao

   Article

   Yang et al Nat Microbiol. 2021 May;6(5):584-593. do

   Insert

   FtsW-tagRFP (ftsW )
   

   Use

   Tags

   tagRFP

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS18

   Plasmid

   #158787

   Purpose

   Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans

   Depositor

   Jennifer Garrison, Zachary Knight

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   Ptag-168::GCaMP6m::rpl-1a
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORANGE Cplx2-GFP KI

   Plasmid

   #131476

   Purpose

   Endogenous tagging of Complexin2: C-terminal (amino acid position: L128)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cplx2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE GFP-CADPS KI

    Plasmid

    #131482

    Purpose

    Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cadps Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE GSG1l-GFP KI

    Plasmid

    #131492

    Purpose

    Endogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gsg1l Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORANGE CACNG8-GFP KI #1

    Plasmid

    #131473

    Purpose

    Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacng8 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pORANGE C9orf4-GFP KI

    Plasmid

    #131472

    Purpose

    Endogenous tagging of C9orf4: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Frrs1l Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAS19

    Plasmid

    #158788

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    psra-6::GCaMP6m::rpl-1a
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS16

    Plasmid

    #158786

    Purpose

    Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Pntc-1::GCaMP6m::rpl-1a
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. SIV_Rev

    Plasmid

    #236244

    Purpose

    3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Rev

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SIV Rev
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. (B) G418 marker (R) (SBE 137)

    Plasmid

    #195011

    Purpose

    geneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+G418+tNOS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXYL

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET19-TdTdelta5cys

    Plasmid

    #207465

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    TdTdelta5cys (Dntt Mouse)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET19-TdTc253

    Plasmid

    #207464

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTc253 (Dntt Mouse)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Ser253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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