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pWP063

Plasmid

#198799

Purpose

Co-expression plasmid for the PHB neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Posm-10-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP026

Plasmid

#198800

Purpose

Co-expression plasmid for the PHB neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrb-6-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP173

Plasmid

#198801

Purpose

Co-expression plasmid for the PHC neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

F58F6.6 (F58F6.6 Nematode)

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP066

Plasmid

#198802

Purpose

Drives specific expression in the URB neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pnlp-48-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP085

Plasmid

#198804

Purpose

Drives specific expression in the URY neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Parrd-16-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP062

Plasmid

#198793

Purpose

Drives specific expression in the IL1 neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Paqp-6-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP068

Plasmid

#198782

Purpose

Drives specific expression in the ASK neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrd-36-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDO010

Plasmid

#198772

Purpose

Drives specific expression in the ALM, PLM, AVM, PVM neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pmec-17-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHW489

Plasmid

#198771

Purpose

Drives specific expression in the ALA neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pflp-24-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP097

Plasmid

#198790

Purpose

Co-expression plasmid for the CEP neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PF09E10.7-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP101

Plasmid

#198791

Purpose

Co-expression plasmid for the FLP, PVD neurons or the PHC neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pkcnl-4-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

Plasmid

#197958

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

CmYLCVp and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_V2_U6_AtPDS3_gRNA10

Plasmid

#197963

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-vCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

AtU6-26 and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsDCP1b_H

Plasmid

#146505

Purpose

Mammalian Expression of HsDCP1b

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1b (DCP1B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP2_G

Plasmid

#146401

Purpose

Mammalian Expression of HsDCP2

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP2 (DCP2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pOPO812

Plasmid

#195306

Purpose

pBAD322-MycaIDCas-ΔCas6-RGR-lacZs, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked lacZ spacer 5.

Depositor

Joseph Peters

Article

Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

Insert

McCAST Cascade Δcas6 and ribozyme flanked lacZ spacer 5

Expression

Bacterial

Promoter

Arabinose inducible arapBAD

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-CBS (H65R)

Plasmid

#182924

Purpose

Mammalian expression plasmid for human CBS mutant H65R

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

Cystathionine Beta-Synthase (CBS Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed histidine 65 to arginine

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R280A,K284A]

Plasmid

#188598

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R280A [CGA=>GCA], K284A [AAG=> GCG]

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[K216E]

Plasmid

#188600

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K216E [AAA=> GAA]

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[C399-625 CM]

Plasmid

#188588

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler C-terminal domain, catalytic mutant

Tags

FLAG-Myc

Expression

Insect

Mutation

D435A [GAT=>GCC]; E435A [GAA=>GCC]

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[C399-625]-Gwpep(Mm)

Plasmid

#188589

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler

Tags

FLAG-Myc and GWpep mm

Expression

Insect

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[K276A]

Plasmid

#188597

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K276A [AAG=>GCG]

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr[C399-625]-Gwpep(Dm)

Plasmid

#188590

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler

Tags

FLAG-Myc and Gwpep dm

Expression

Insect

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Gwpep(Dm)-Nbr[C399-625]

Plasmid

#188592

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler

Tags

FLAG-Myc-Gwpep dm

Expression

Insect

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Gwpep(Mm)-Nbr[C399-625]

Plasmid

#188591

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler

Tags

FLAG-Myc-Gwpep mm

Expression

Insect

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr[R272A,K276A]

Plasmid

#188596

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG], K276A [AAG=>GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[K284A]

Plasmid

#188595

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K284A [AAG=> GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr[R272A]

Plasmid

#188594

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr[K187A]

Plasmid

#188593

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K187A [AAA=> GCA]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr

Plasmid

#188584

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

deleted first ATG

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge