Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,585 results for: BLI

Clear keywords
Showing: 2681 - 2700 of 9585 results

  1. eSpCas9(1.1)-T2A-Puro

    Plasmid
    #101039
    Purpose
    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.
    Depositor
    Andrea Németh
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3xFLAG and T2A-Puro
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    101039-plasmid-map-sequence-id-190363

    Enlarge

  2. pExp-DsbC

    Plasmid
    #112564
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-DsbC and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112564-plasmid-map-sequence-id-217214

    Enlarge

  3. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

    Enlarge

  4. pcDNA3.1-3'3xFlag

    Plasmid
    #208616
    Purpose
    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208616-plasmid-map-sequence-id-418312

    Enlarge

  5. pTCF156 HLA-A*02:01

    Plasmid
    #180456
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180456-plasmid-map-sequence-id-355799

    Enlarge

  7. pCLIP-tag (m) Vector

    Plasmid
    #101136
    Purpose
    Expression of protein of interest with CLIP-Tag in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Tags
    CLIP-tag (CLIP10)
    Expression
    Mammalian
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101136-plasmid-map-sequence-id-194177

    Enlarge

  8. pcDNA-DDX53-FLAG

    Plasmid
    #222241
    Purpose
    Express FLAG tagged DDX53 in mammalian cells
    Depositor
    Yuliang Wu
    Insert
    DDX53 (DDX53 Human)
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    T7
    Available Since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    222241-plasmid-map-sequence-id-441350

    Enlarge

  9. pcDNA-DDX53-GFP

    Plasmid
    #222239
    Purpose
    Express GFP tagged DDX53 in mammalian cells
    Depositor
    Yuliang Wu
    Insert
    DDX53 (DDX53 Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    222239-plasmid-map-sequence-id-441404

    Enlarge

  10. pJA54 HLA-A*03:01

    Plasmid
    #180451
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*03:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    180451-plasmid-map-sequence-id-355758

    Enlarge

  11. SPLICSFAST-ER-MT-P2A

    Plasmid
    #214892
    Purpose
    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-ER-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214892-plasmid-map-sequence-id-423478

    Enlarge

  12. pR6K-GFP-CASTIF

    Plasmid
    #199653
    Purpose
    R6K plasmid encoding a type I-F CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST I-F systems
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199653-plasmid-map-sequence-id-412253

    Enlarge

  13. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh and U6
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    91854-plasmid-map-sequence-id-176529

    Enlarge

  14. pLIX403-EYFP-ccdB-Hygro

    Plasmid
    #158555
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158555-plasmid-map-sequence-id-309868

    Enlarge

  15. pTCF158 Human beta-2 microglobulin

    Plasmid
    #180457
    Purpose
    E. coli expression of class I MHC light chain
    Depositor
    John Altman
    Insert
    Human beta-2 microglobulin
    Expression
    Bacterial
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180457-plasmid-map-sequence-id-355801

    Enlarge

  16. pPB-CAG-Venus-Akaluc

    Plasmid
    #209768
    Purpose
    Venus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Yohei Hayashi
    Insert
    Venus-Akaluc
    Use
    Luciferase; Piggybac transposon
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    209768-plasmid-map-sequence-id-417214

    Enlarge

  17. AAV-CAG-jGCaMP8f-WPRE

    Plasmid
    #179254
    Purpose
    AAV-mediated expression of GCaMP8f under the CAG promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8f
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179254-plasmid-map-sequence-id-381183

    Enlarge

  18. PB CAG-mTagBFP2-T2A-iCreERT2

    Plasmid
    #177308
    Purpose
    CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    mTagBFP2-T2A-iCreERT2
    Use
    Cre/Lox; Piggybac
    Expression
    Mammalian
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177308-plasmid-map-sequence-id-348855

    Enlarge

  19. pE-SUMO CRYGD

    Plasmid
    #80755
    Purpose
    Expression of human gamma D-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGD (CRYGD Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80755-plasmid-map-sequence-id-153570

    Enlarge

  20. pRha-ABE8e-NRCH

    Plasmid
    #165417
    Purpose
    For bacterial purification of ABE8e-NRCH protein
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Tags
    8xHIS and BP-NLS
    Expression
    Bacterial
    Promoter
    pRhaBAD
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165417-plasmid-map-sequence-id-334014

    Enlarge

Showing: 2681 - 2700 of 9585 results