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pET30-T7-His-sfGFP

Plasmid

#180755

Purpose

pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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PBS Actb-2A-TIR1-GFP

Plasmid

#113832

Purpose

targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

osTIR1-GFP (NEWENTRY Synthetic)

Use

Synthetic Biology

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-MBP1-E322TAG-W340A.pcDNA3-k

Plasmid

#127404

Purpose

Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.

Depositor

Sharona Gordon

Article

Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

Insert

Maltose Binding Protein

Tags

FLAG

Expression

Mammalian

Mutation

Amino acid mutations: E322 to Amber codon and W34…

Promoter

CMV

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Foxa2

Plasmid

#33004

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A2 (Foxa2 Mouse)

Use

Retroviral

Available Since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only
Filamin-C-stFRET

Plasmid

#61107

Purpose

Expression of Filamin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Hbxadr-Hatag

Plasmid

#24930

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Tags

HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pX330A-1x7

Plasmid

#58771

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-siRNA control

Plasmid

#10900

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siGFP

Expression

Mammalian

Available Since

Feb. 17, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT/TO MycLAP-STIL

Plasmid

#80266

Purpose

mammalian expression plasmid for MycLAP-STIL

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

STIL (STIL Human)

Tags

Myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSL1394

Plasmid

#129522

Purpose

pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9

Use

Plasmodium, rodent-infectious species

Tags

3XFLAG-NLS

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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LifeAct-scramble

Plasmid

#217499

Purpose

Expresses LifeAct-mScarlet-scramble to serve as control plasmid for LifeAct-MTED

Depositor

Erik Dent

Article

Holland et al Mol Biol Cell. 2024 Jun 1;35(6):mr3. doi

Insert

LifeAct-mScarlet-scramble

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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p38delta

Plasmid

#29575

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

p38delta (Mapk13 Mouse)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Starting Met replaced with Ala

Available Since

July 13, 2011

Availability

Academic Institutions and Nonprofits only
pTH-MYCN

Plasmid

#35416

Depositor

William A. Weiss

Article

Weiss et al EMBO J. 1997 Jun 2;16(11):2985-95.

Insert

v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN Human)

Expression

Mammalian

Promoter

Tryosine Hydroxlyase (TH)

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

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pRetroSuper Smad3

Plasmid

#15726

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad3 shRNA (SMAD3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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pG12-Rluc8

Plasmid

#170272

Purpose

Renilla reniformisluciferase (RLuc8) expression in mammlian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

RLuc8

Expression

Mammalian

Promoter

12xUAS with TATA-box minimal promoter

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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mOrange pHis-//

Plasmid

#53304

Purpose

Empty vector to express N-terminal His-mOrange-tagged fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Type

Empty backbone

Tags

His and mOrange

Expression

Bacterial

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCJH027_SC101_pLAC_LbCas12a_Kan

Plasmid

#183074

Purpose

Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a

Expression

Bacterial

Mutation

Wild-type

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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p153 pPr-cre

Plasmid

#8403

Purpose

Expression of Cre from tissue-specific, androgen-inducible probasin promoter.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

probasin driven Cre

Use

Cre/Lox

Expression

Mammalian

Promoter

probasin promoter

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
lenti-fullef1a-mutFAKSPARK2

Plasmid

#195266

Purpose

express Tyr to Phe mutant FAKSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

Tyr to Phe mutant FAKSPARK2 sensor

Use

Lentiviral

Expression

Mammalian

Promoter

ef1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp8a

Plasmid

#163592

Purpose

Expresses BMP-8A in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp8a (Bmp8a Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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