We narrowed to 3,462 results for: mpo

1. pcDNA3-N-Flag-NLRP3 91-710

   Plasmid

   #75140

   Purpose

   Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 91-710

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

   Plasmid

   #169161

   Purpose

   To express nsp10-nsp14 fusion protein in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1

   Plasmid

   #52504

   Purpose

   mammalian expression vector of BRCA1 wildtype siRNA resistant

   Depositor

   Daniel Durocher

   Article

   Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   siRNA resistant sequence: AGTATAATC

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLEVI(408)-ColE-iLight-msfGFP

   Plasmid

   #170268

   Purpose

   Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   iLight
   

   Use

   Tags

   msfGFP

   Expression

   Bacterial

   Mutation

   To design iLight for expression in bacteria, a fu…

   Promoter

   pJ23116

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-NanoLuc-BRAF-CAT

   Plasmid

   #194772

   Purpose

   Mammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.

   Depositor

   Deborah Morrison

   Article

   Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

   Insert

   BRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)
   

   Use

   Tags

   NanoLuc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-BRAF-REG-Halo

   Plasmid

   #194771

   Purpose

   Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.

   Depositor

   Deborah Morrison

   Article

   Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

   Insert

   BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. R725-X11-566: His6-MBP-tev-Hs.LGALS3

   Plasmid

   #159699

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.LGALS3

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Hs.LGALS3 (LGALS3 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV TRE3G Neo GFP-Progerin C661S

   Plasmid

   #118711

   Purpose

   Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)

   Depositor

   Tom Misteli

   Article

   Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

   Insert

   GFP-Progerin C661S (LMNA Human)
   

   Use

   Lentiviral; Tet-on inducible

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C661S farnesylation mutant

   Promoter

   CMV TRE3G (TET-ON)

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-Tet-O-RUNX1T1-Puro

   Plasmid

   #221666

   Purpose

   Expresses RUNX1T1 in mammalian cells

   Depositor

   Vanessa Hall

   Article

   Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

   Insert

   RUNX1T1 (RUNX1T1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

    Plasmid

    #169160

    Purpose

    To express nsp14-nsp10 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

    Plasmid

    #159700

    Purpose

    E. coli protein expression

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)
    

    Use

    Tags

    His6-MBP-tev-Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET11a_3xFlag-nsp14 (SARS-CoV-2)

    Plasmid

    #169159

    Purpose

    To express SARS-CoV-2 nsp14 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)

    Plasmid

    #169189

    Purpose

    Baculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    3xFlag-His6-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

    Plasmid

    #169185

    Purpose

    Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    His6-3xFlag (nsp12 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQCXIZ GFP PODXL delta PBM + FBM

    Plasmid

    #202416

    Purpose

    Expression of GFP-tagged PODXL PDZ-binding and FERM-binding motif mutation

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Lacking DTHL, and H477/R479/S481>AAA mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid

    #169180

    Purpose

    Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp7-His6-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    His6 (internal tag linking nsp7 and nsp8): nsp7-H…

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQCXIZ GFP PODXL FKBP 4K>R

    Plasmid

    #202421

    Purpose

    Expression of ubiquitination-deficient GFP-tagged PODXL with FKBP-rapamycin binding (FRB) dimerization domain

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    FKBP and GFP

    Expression

    Mutation

    K431/525/526/547R

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid

    #169184

    Purpose

    Baculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    His6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid

    #169183

    Purpose

    Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-3xFlag/nsp7-His6-nsp8 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag (nsp12 C-terminus) and His6 (as internal t…

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET11a_3xFlag-nsp10 (SARS-CoV-2)

    Plasmid

    #169157

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VRLQ]-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    3xFlag and nsp5CS[VRLQ]: predicted nsp5 protease …

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge