We narrowed to 14,304 results for: TIM;

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pKM45

Plasmid

#154152

Purpose

Firefly luciferase with no promoter for negative control for luciferase assays

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pYFP-lacY-10

Plasmid

#140139

Purpose

Evaluating the impact of feedback and high sensor concentration on information transmission of a genetic sensor.

Depositor

Drew Tack

Article

Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

Insert

lacY (lacY )

Expression

Bacterial

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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FoxFTVC+bpFOG_Cdkn1b.a

Plasmid

#107523

Purpose

Negative regulator of cell cycle progression by G1/S transition inhibition

Depositor

Lionel Christiaen

Article

Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

Insert

Cdkn1b.a

Promoter

TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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FoxFTVC+bpFOG_Cdkn1b.b

Plasmid

#107524

Purpose

Negative regulator of cell cycle progression by G1/S transition inhibition

Depositor

Lionel Christiaen

Article

Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

Insert

Cdkn1b.b

Promoter

TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGuide-P1-O1a (EL702)

Plasmid

#140040

Purpose

CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.

Depositor

Johan Elf

Article

Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:

Insert

barcode P1 and sgRNA lacO1 array

Use

Synthetic Biology

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCCG 306

Plasmid

#131167

Purpose

PCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Tags

CCG

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFH70

Plasmid

#128190

Purpose

Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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IgH intron enhancer

Plasmid

#127247

Purpose

To maximize gene expression in B cells

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

heavy chain enhancer

Expression

Mammalian

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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KA2963_eSpCas9-PGKHygdtkCh

Plasmid

#124205

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2

Plasmid

#124169

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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KA1123_pPB-TREtight-cHA-IRESH2BmKO2

Plasmid

#124172

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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KFP14

Plasmid

#119796

Purpose

RpoD6 epitope tagged construct for integration at neutral site 1

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1557 (Synpcc7942_1557 )

Expression

Bacterial

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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KFP13

Plasmid

#119795

Purpose

RpoD5 epitope tagged construct for integration at neutral site 1

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1849 (Synpcc7942_1849 )

Expression

Bacterial

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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KFP06

Plasmid

#119799

Purpose

RpoD6 epitope tagged construct for integration at neutral site 2.2

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1557 (Synpcc7942_1557 )

Expression

Bacterial

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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KFP05

Plasmid

#119800

Purpose

RpoD5 epitope tagged construct for integration at neutral site 2.2

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1849 (Synpcc7942_1849 )

Expression

Bacterial

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pHES945

Plasmid

#112643

Purpose

blue light inducible cryptochrome based optogenetic expression system

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

CRY2PHR-GAL4DBD

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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ReNL-Actin_pcDNA3

Plasmid

#89531

Purpose

Red color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pVHed07

Plasmid

#82350

Purpose

Bxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmid

Depositor

Richard Murray

Article

Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

Inserts

mKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETb-pcDronpa

Plasmid

#78183

Purpose

Expresses pcDronpa in E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

pcDronpa

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pNB762

Plasmid

#60150

Purpose

Firefly luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LEU1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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