We narrowed to 16,170 results for: Pol;

1. pAAV-CAG-SuperNovaGreen-T2A-dTomato

   Plasmid

   #193798

   Purpose

   Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   SuperNova Green
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-miniSOG-T2A-dTomato

   Plasmid

   #193796

   Purpose

   Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCV-Rep-

   Plasmid

   #32058

   Depositor

   Patrick Moore

   Article

   Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

   Insert

   MCV-Rep- genome
   

   Mutation

   Single cytosine to adenine (C/A) mutation at 2,07…

   Promoter

   none from vector. Insert contains native promoter…

   Available Since

   Oct. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWzyE-His

   Plasmid

   #196669

   Purpose

   Expression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coli

   Depositor

   James Naismith

   Article

   Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

   Insert

   WzyE
   

   Tags

   TEV cleavable His10

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDestTol2pA ins:R198P mVenus

   Plasmid

   #210519

   Purpose

   Expresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

   Depositor

   Jonathan Rocheleau

   Article

   Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

   Insert

   Apollo-NADP+
   

   Use

   Zebrafish expression, tol2 kit gateway-compatible

   Tags

   mVenus

   Mutation

   R198P

   Promoter

   Zebrafish insulin promoter

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-fsr1

   Plasmid

   #179866

   Purpose

   Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiae

   Depositor

   Jens Laurids Sørensen

   Article

   Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

   Insert

   Fusarubin polyketide synthase
   

   Use

   Synthetic Biology

   Mutation

   Codon optimized for expression in Saccharomyces c…

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-miniSOG-C-Far

   Plasmid

   #193914

   Purpose

   Control plasmid that expresses miniSOG targeted to the cell membrane in mammalian cells

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Farnesylation signal sequence and Flag tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. H1.224.luc

   Plasmid

   #193704

   Purpose

   Expresses a truncated PolII H1 promoter (H1.244)

   Depositor

   Elena Herrera-Carrillo

   Article

   Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

   Insert

   Truncated PolIII H1 promoter of 224 nucleotides (H1.224) + N41 fragment
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-2T-RNAPII-A2-14-HA

   Plasmid

   #160689

   Purpose

   Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains an S-to-A mutation in position 2 of 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

   Depositor

   Jeffry Corden

   Article

   Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

   Insert

   RNA polymerase II CTD
   

   Expression

   Bacterial

   Mutation

   Contains an S-to-A mutation in position 2 of 14 o…

   Available Since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid

    #156432

    Purpose

    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-N BKV ER

    Plasmid

    #37864

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    BKV ER (BKPyVgp5, BKPyVgp6 BK polyomavirus)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

12. CAG-mCherry

    Plasmid

    #108685

    Purpose

    Strong CAG promoter with mCherry expression for transfection efficacy screening

    Depositor

    Jordan Green

    Article

    Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

    Plasmid

    #156440

    Purpose

    For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA1 (Stag1 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p(A)n-PAUSE

    Plasmid

    #118068

    Purpose

    Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

    Plasmid

    #156445

    Purpose

    For transient expression of mouse RAD21 (SCC1) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse RAD21 (Rad21 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

    Plasmid

    #156436

    Purpose

    For transient expression of human PDS5B tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    human PDS5B (PDS5B Human)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

    Plasmid

    #156441

    Purpose

    For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA2 (Stag2 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

    Plasmid

    #139446

    Purpose

    Lentiviral vector genome to ectopically express Firefly luciferase

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag Firefly luciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH21-BNA-Luc

    Plasmid

    #187924

    Purpose

    Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
    

    Expression

    Mammalian

    Promoter

    RNA polymease I Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187918

    Purpose

    Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 1
    

    Tags

    3xFLAG

    Promoter

    CMV Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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