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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 2701 - 2720 of 7951 results

  1. pYPQ239-RR

    Plasmid
    #108862
    Purpose
    FnCpf1 Gateway entry plasmid with N623R and K687R mutations
    Depositor
    Yiping Qi
    Article
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    FnCpf1-RR (FnCpf1 with N623R and K687R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N623R and K687R, Cpf1 is rice codon optimized
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108862-plasmid-map-sequence-id-274177

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  4. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

    Plasmid
    #133793
    Purpose
    U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    CjeCas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    V2-TGAA linker sgRNA architecture from Kim et al.…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133793-plasmid-map-sequence-id-268077

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  15. pETDuet-msGC-NT21

    Plasmid
    #78102
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 2…
    Promoter
    T7
    Available Since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    78102-plasmid-map-sequence-id-151428

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  16. pETDuet-msGC-NT13

    Plasmid
    #75087
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 4…
    Available Since
    May 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    75087-plasmid-map-sequence-id-148282

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  17. pKK-mVenus-TEV

    Plasmid
    #105774
    Purpose
    Expression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mVenus-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105774-plasmid-map-sequence-id-203363

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  18. pGA1-EF1α-sfGFP150TAG-4xtRNA

    Plasmid
    #251517
    Purpose
    Expression of sfGFP150TAG using the Methanomethylophilus alvus (Ma) PylRS/tRNA system for site-specific incorporation of noncanonical amino acids at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021
    Insert
    sfGFP150TAG
    Tags
    His6 and V5
    Expression
    Mammalian
    Mutation
    150 TAG (amino acid 150 mutated to stop codon)
    Promoter
    EF1alpha
    Available Since
    March 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    251517-plasmid-map-sequence-id-502878

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  20. pCR8-CDK12

    Plasmid
    #208347
    Purpose
    Gateway cloning entry vector with CDK12. Must be recombined into a DEST vector for expression.
    Depositor
    Peter S Nelson
    Article
    Frank et al eLife 2025
    Insert
    cyclin dependent kinase 12 (CDK12 Human)
    Use
    Gateway: entry vector
    Available Since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    208347-plasmid-map-sequence-id-416919

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Showing: 2701 - 2720 of 7951 results