We narrowed to 10,108 results for: SIM;

1. pAD-LyseR (in lacZ deficient strain)

   Plasmid

   #99245

   Purpose

   For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   phage lambda gene R (R phage lambda)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   beta-lactamase promoter

   Available since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCyRFP1-RhoA

   Plasmid

   #84358

   Purpose

   fusion protein of RhoA, FRET donor

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mCyRFP1-RhoA (RHOA Human, Synthetic)
   

   Use

   Tags

   mCyRFP1 N terminal fusion to RhoA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScalps_puro_mIL-2Rα

   Plasmid

   #59917

   Purpose

   Expresses mouse interleukin-2 receptor alpha chain

   Depositor

   Silvia Monticelli

   Article

   Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

   Insert

   interleukin 2 receptor alpha (Il2ra Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pSR651

   Plasmid

   #65213

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-1 cap
   

   Use

   AAV

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10 and polyhedrin

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C2_SEPT9 i1

   Plasmid

   #71609

   Purpose

   mammalian expression of human SEPT9 i1 with a GFP fusion.

   Depositor

   Elias Spiliotis, Cristina Montagna

   Article

   Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

   Insert

   Septin 9 (SEPTIN9 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMJ923

   Plasmid

   #78313

   Purpose

   Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

   Depositor

   Martin Jinek

   Article

   Burger et al Development. 2016 Apr 29. pii: dev.13480

   Insert

   SpCas9
   

   Use

   Tags

   HA-2xNLS-mCherry-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   human codon-optimized gene sequence

   Promoter

   T7

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2TAL3-RR

   Plasmid

   #37276

   Purpose

   TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-term tal protein segments increases mutation induction; Fok I RR (improved version = pCS2TAL3-RRR)

   Depositor

   David Grunwald

   Article

   Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

   Type

   Empty backbone

   Use

   Tags

   C terminal Tal From pTAL3, FokI RR, HA, and N-ter…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-IGK-dAPEX2-KDEL

   Plasmid

   #117182

   Purpose

   Plasmid name in publication: pAAV-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   IGK-dAPEX2-KDEL
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C2_SEPT9 i4

   Plasmid

   #71617

   Purpose

   mammalian expression of human SEPT9 i4 with a GFP fusion

   Depositor

   Elias Spiliotis, Cristina Montagna

   Article

   Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

   Insert

   Septin 9 (SEPTIN9 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBRPyCAG-hKlf2-DsRed-Ip

    Plasmid

    #26277

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Klf2 (KLF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQTEV-HSPB8

    Plasmid

    #34710

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    heat shock 22 kDa protein 8 (HSPB8 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FLAG-ARP8

    Plasmid

    #15361

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    actin-related protein 8 (ACTR8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV CaMKIIa iChloC 2A tDimer

    Plasmid

    #66709

    Purpose

    Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E83Q,E90R,E101S,D156N,T159C

    Promoter

    CaMKIIa

    Available since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQStrep2-ARPC3

    Plasmid

    #31580

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    Actin related protein 2/3 complex, subunit 3 (ARPC3 Human)
    

    Use

    Tags

    His and StrepII-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-FDIO-COX4-dAPEX2

    Plasmid

    #117178

    Purpose

    Plasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    COX4-dAPEX2
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lenti-SAM-hygro

    Plasmid

    #138955

    Purpose

    Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

    Depositor

    Nadya Dimitrova

    Article

    Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 AND EF1a

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HOXB4 (human) pSG5

    Plasmid

    #8527

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    In vitro transcriptioin/translation

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pQTEV-MAP1LC3A

    Plasmid

    #34824

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    microtubule-associated protein 1 light chain 3 alpha (MAP1LC3A Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FCK-ArchT-GFP

    Plasmid

    #31177

    Purpose

    Lentiviral expression of ArchT-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-FDIO-LACTB-dAPEX2

    Plasmid

    #117181

    Purpose

    Plasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    LACTB-dAPEX2
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    W41F and A134P on soybean APX

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge