We narrowed to 5,085 results for: mcs

1. pMSCV-hygro-STING HAQ+V155M

   Plasmid

   #102601

   Purpose

   Express STING V55M HAQ for IFN-beta Luciferase reporter assay.

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   V155M R71H-G230A-R293Q

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. C1ORF43-3xFLAG

   Plasmid

   #130880

   Purpose

   Expresses human C1ORF43 with C-terminal 3xFLAG tag

   Depositor

   Michael Bassik

   Article

   Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

   Insert

   C1ORF43 (C1orf43 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDuEx2-NIR-GECO1

   Plasmid

   #113680

   Purpose

   Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

   Depositor

   Robert Campbell

   Article

   Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

   Insert

   NIR-GECO1
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIRCgp41-1

   Plasmid

   #74227

   Purpose

   Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   gp41-1 C-intein and gp41-1 N-intein

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. OPEN HLA-A*02:01-BirA

   Plasmid

   #215569

   Purpose

   E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN HLA-A*02:01 (HLA-A Human)
   

   Tags

   BirA

   Expression

   Bacterial

   Mutation

   Glycine 120 to Cysteine

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. yeast_DualReporter

   Plasmid

   #127546

   Purpose

   A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).

   Depositor

   Aviv Regev

   Article

   de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do

   Inserts

   bla
   mCherry
   URA3 (URA3 Budding Yeast)
   yEVenus
   Nat1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-RAB10 T23N

   Plasmid

   #130885

   Purpose

   Expresses human RAB10 T23N mutant with N-terminal GFP tag

   Depositor

   Michael Bassik

   Article

   Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

   Insert

   RAB10 (RAB10 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T23N

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. OPEN-HLA-B*07:02-BirA

   Plasmid

   #239752

   Purpose

   E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-B*07:02 (HLA-B Human)
   

   Tags

   BirA substrate peptide

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   T7

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Rab27B Q78L

   Plasmid

   #89448

   Purpose

   Expression of GFP-tagged human Rab27B Q78L in mammalian cells

   Depositor

   Wendy Westbroek

   Article

   Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

   Insert

   human Rab27B Q78L (RAB27B Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Q78L

   Promoter

   CMV

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV 4xSTAR-mTagBFP2-blast

    Plasmid

    #125239

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    mTagBFP2 (codon optimized)

    Expression

    Mammalian

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Myc-AIM2

    Plasmid

    #73958

    Purpose

    Express c-Myc-tagged human AIM2 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

    Insert

    absent in melanoma 2 (AIM2) (AIM2 Human)
    

    Tags

    c-myc

    Expression

    Mammalian

    Promoter

    CMV iE

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIRCNpu

    Plasmid

    #74226

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Npu DnaE C-intein and Npu DnaE N-intein

    Expression

    Bacterial

    Promoter

    T7-lac

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiGuide-Crimson

    Plasmid

    #70683

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    S. pyogenes sgRNA cassette
    Crimson Reporter
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Ef1-a and hU6

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetroX-Tight_A-CREB_PGK-tdTomato

    Plasmid

    #154264

    Purpose

    Dox/Tet-inducible expression of dominant negative CREB (A-CREB) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    dominant negative CREB
    

    Expression

    Mammalian

    Promoter

    Tight/tetO

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-Rab27B N133I

    Plasmid

    #89449

    Purpose

    Expression of GFP-tagged human Rab27B N133I in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B N133I (RAB27B Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    N133I

    Promoter

    CMV

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-Rab27B T23N

    Plasmid

    #89450

    Purpose

    Expression of GFP-tagged human Rab27B T23N in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B T23N (RAB27B Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    T23N

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIZ-EGFP-MYO10-HMM

    Plasmid

    #87257

    Purpose

    Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Expression

    Insect

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    OpIE2

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTwist-SARS-CoV Δ18

    Plasmid

    #169465

    Purpose

    Encodes SARS-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE-HA-GOLPH3

    Plasmid

    #21687

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. VRK1-dTag-puro

    Plasmid

    #199651

    Purpose

    Expresses VRK1 C-term. Tagged with dTAG

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK1 (VRK1 Human)
    

    Use

    Lentiviral

    Tags

    dTAG

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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