We narrowed to 3,466 results for: ttl

1. pYPQ166

   Plasmid

   #109328

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJAK184

   Plasmid

   #167281

   Purpose

   MazF-based mutagenesis vector for C. difficile

   Depositor

   Robert Fagan

   Article

   Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

   Type

   Empty backbone

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Pxyl

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pdCASclos

   Plasmid

   #73640

   Purpose

   Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   dCas9
   sgRNA to spo0A
   

   Use

   E.coli-clostridium shuttle vector

   Tags

   Expression

   Mutation

   D10A, H840A

   Promoter

   Pj23119 and ptb

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS1ClacZ

   Plasmid

   #55171

   Purpose

   lacZ-reporter vector, integration at amyE, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pDGE476

   Plasmid

   #153267

   Purpose

   M2E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCast

   Plasmid

   #200169

   Purpose

   E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

   Depositor

   Yong-Su Jin

   Article

   Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAD-SPS6-Pman

   Plasmid

   #133906

   Purpose

   Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(SPS6)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pman

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYPQ230-E795L

   Plasmid

   #176890

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

   Insert

   LbCas12a-E795L
   

   Use

   CRISPR; Gateway compatible lbcpf1 entry clone

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. attB53-Pac-TRE-mCherry-attB53

   Plasmid

   #183611

   Purpose

   RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   TRE-mCherry
   

   Use

   Rmce

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAD-mKate(KPS12)

    Plasmid

    #133831

    Purpose

    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    mKate(KPS12)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pupp

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDGE657

    Plasmid

    #153255

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOCC65

    Plasmid

    #118865

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, mGFP

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXD70DA9-Pct5-DadhABC(A2)

    Plasmid

    #191632

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGE479

    Plasmid

    #153270

    Purpose

    M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEG R3-miRNA(ccdB)-L4 #BGV228

    Plasmid

    #48953

    Purpose

    To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    na

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pJAK080

    Plasmid

    #167279

    Purpose

    CodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficile

    Depositor

    Robert Fagan

    Article

    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

    Type

    Empty backbone

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221 CARD8

    Plasmid

    #169962

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBsVchCAST

    Plasmid

    #203812

    Purpose

    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

    Depositor

    Sheng Yang

    Article

    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

    Insert

    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC292

    Plasmid

    #118881

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    mRuby3, HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOCC291

    Plasmid

    #118867

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, mRuby3

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge