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Plasmid#118908Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tagDepositorInsertNcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
UseTagsYBBR tag, HIS10, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-MCS (JDW 455)
Plasmid#229832PurposeA gateway compatible middle entry clone containing an MCS (mutiple cloning site).DepositorInsertattL1/L2 flanked MCS
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262F-ABE8e
Plasmid#199179PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e-HF mediated A-G base editingDepositorInsertABE8e(V106W)-zSpCas9(D10A)
UseCRISPR; Gateway compatible abe8e(v106w)-zspcas9(d…TagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3
Plasmid#201534PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB3
UseSynthetic BiologyTagsExpressionMutationrhaS W190GPromoterPrhaBAD (rhamnose)Available sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCgVchCAST-crtYf
Plasmid#203816PurposeExpression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCgVchDonor
Plasmid#203817PurposeDNA donor for transposition, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertMini-Tn, Spec cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ7-DadR/DadS-Pdadh-LacZ
Plasmid#191622PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ with transcriptional regulators DadR/DadSDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ8-CadR-Pcadh-LacZ
Plasmid#191623PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadRDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ9.1-HcdR-Phcdh-LacZ
Plasmid#191629PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ with transcriptional regulator HcdRDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadRmt4)-DadR(linker)
Plasmid#191639PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(linker region shuffled) complementationDepositorInsertDadR
UseTagsExpressionBacterialMutationLinker region sequence shuffledPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadR-NFLAG)-N-3xFLAG-DadR
Plasmid#191640PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR complementationDepositorInsertDadR
UseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadRmt5)-N-3xFLAG-DadR(ΔDBD)
Plasmid#191641PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔDBD) complementationDepositorInsertDadR
UseTags3xFLAGExpressionBacterialMutationΔDBDPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadRmt6)-N-3xFLAG-DadR(ΔTM)
Plasmid#191642PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔTM) complementationDepositorInsertDadR
UseTags3xFLAGExpressionBacterialMutationΔTMPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)
Plasmid#191643PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementationDepositorInsertDadR
UseTags3xFLAGExpressionBacterialMutationLinker region sequence shuffledPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadH)-Pdadh-DadhABC(A2)
Plasmid#191644PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265N2
Plasmid#174001PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-SpRY-UNG
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265O2
Plasmid#174002PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265L2
Plasmid#174000PurposeGateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editingDepositorInsertUNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationIn rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66
Plasmid#168787PurposeDonor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.DepositorTypeEmpty backboneUseCre/Lox and Synthetic Biology; Expression in fila…TagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 ZUC
Plasmid#169964PurposepDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only