We narrowed to 3,491 results for: ttl

1. pDGE497

   Plasmid

   #153249

   Purpose

   M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR E3 pR8A L3-L2

   Plasmid

   #158735

   Purpose

   Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NN)

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   TALEN ORF without TAL repeat
   

   Use

   TALEN

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR E3 pR5A L3-L2

   Plasmid

   #158732

   Purpose

   Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   TALEN ORF without TAL repeat
   

   Use

   TALEN

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE342

   Plasmid

   #153251

   Purpose

   M8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR E3 pR6A L3-L2

   Plasmid

   #158733

   Purpose

   Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NG)

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   TALEN ORF without TAL repeat
   

   Use

   TALEN

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR E3 pR7A L3-L2

   Plasmid

   #158734

   Purpose

   Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NI)

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   TALEN ORF without TAL repeat
   

   Use

   TALEN

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEC3006 (pSpy1C-PZn_ffluc)

   Plasmid

   #218515

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PZn (PczcD)

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPDK3860

   Plasmid

   #199245

   Purpose

   Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
   

   Use

   T-dna vector

   Promoter

   pPEBV

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNW-sfGFP(SPS6)

   Plasmid

   #133814

   Purpose

   Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(SPS6)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Expression

   Bacterial

   Promoter

   Ppta

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS4S

    Plasmid

    #55170

    Purpose

    Empty vector, integration at thrC, ampr, specr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pNICKclos1.0

    Plasmid

    #73639

    Purpose

    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sGRNA to pyrE
    

    Use

    E.coli-clostridium shuttle vector

    Mutation

    D10A

    Promoter

    j23119 and ptb

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXD70LacZ6-LacI-Ptac(LacO)-LacZ

    Plasmid

    #191621

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ166

    Plasmid

    #109328

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBSV2G_P0826-BB0323-mCherryBb

    Plasmid

    #118244

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    BB0323-mCherry translational fusion under the control of the bb0826 promoter
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJAK184

    Plasmid

    #167281

    Purpose

    MazF-based mutagenesis vector for C. difficile

    Depositor

    Robert Fagan

    Article

    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

    Type

    Empty backbone

    Use

    E. coli - c. difficile shuttle vector

    Promoter

    Pxyl

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ

    Plasmid

    #191628

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdCASclos

    Plasmid

    #73640

    Purpose

    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    dCas9
    sgRNA to spo0A
    

    Use

    E.coli-clostridium shuttle vector

    Mutation

    D10A, H840A

    Promoter

    Pj23119 and ptb

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS1ClacZ

    Plasmid

    #55171

    Purpose

    lacZ-reporter vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    none

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCast

    Plasmid

    #200169

    Purpose

    E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

    Depositor

    Yong-Su Jin

    Article

    Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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