Addgene

We narrowed to 13,108 results for: Mpl;

Showing: 2741 - 2760 of 13108 results

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pJAT18

Plasmid

#204307

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-GFPn

Use

CRISPR

Tags

Expression

Mutation

Promoter

MHC

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Rabaptin-5

Plasmid

#199173

Purpose

Expresses GFP-Rabaptin-5 fusion in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

Rabaptin-5 (RABEP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains Tyr 149 Ser substitution; insert starts …

Promoter

CMV

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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mSA-phi29

Plasmid

#210815

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

mSA-Phi29

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBEVY-L

Plasmid

#51226

Purpose

bi-directional expression vector for yeast, constitutively active, LEU2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD/ADH1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

Plasmid

#55221

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

StrepII and msfGFP

Expression

Insect

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pMaCTag-07

Plasmid

#124786

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Expression

Mutation

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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SchBE4max

Plasmid

#192135

Purpose

Expresses AncBE4max and nSchCas9

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

AncBE4max, nSchCas9

Use

Tags

Expression

Mammalian

Mutation

SchCas9 (D9A)

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGagMBPHis

Plasmid

#42176

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

Gag protein sequence is from HIV-1 clone NL4-3

Use

Tags

6xHIS and MBP

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
p6899 PHAGE-P CMVT N-HA-hE6AP II C843A

Plasmid

#37604

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP II C843A (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C843A

Promoter

CMVt

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
psiCHECK SCN1B 3'UTR

Plasmid

#19855

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

SCN1B 3'UTR (SCN1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKD3.RNAi

Plasmid

#10818

Depositor

Alex Toker

Article

Hausser et al Nat Cell Biol. 2005 Sep . 7(9):880-6.

Insert

PKD3 RNAi (PRKD3 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pscALPS_puro

Plasmid

#128504

Purpose

Encodes puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

Puromycin Resistance

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Cyclophilin A promoter

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSTK120-mCMV-TK

Plasmid

#22054

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

mCMV-TK-pA expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pRK5-Myc-TEAD4-Y429H

Plasmid

#33041

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD4 (TEAD4 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Y429H

Promoter

Available since

March 28, 2012

Availability

Academic Institutions and Nonprofits only
pKD016

Plasmid

#207932

Purpose

Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M:FastRed, for braiding

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z31

Plasmid

#120074

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMP118

Plasmid

#207947

Purpose

GreenBraid destination vector pGBY1 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 17, 2023

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2

Plasmid

#88915

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

Cas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pTC201B

Plasmid

#52193

Purpose

for creating reversible targeting construct

Depositor

Tian Chi

Article

Chaiyachati et al BMC Biol. 2012 Nov 30;10:96. doi: 10.118

Insert

promoter-trap cassette Neo-Ires-GFP

Use

Cre/Lox

Tags

Neo, GFP

Expression

Mutation

Promoter

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

Plasmid

#84254

Purpose

Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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