We narrowed to 8,996 results for: ef1;

1. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

   Plasmid

   #187960

   Purpose

   AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SARS_COV2_ORF6_HA_BASU

   Plasmid

   #153984

   Purpose

   Express Orf6 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf6 (ORF6 Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SARS_COV2_ORF8_HA_BASU

   Plasmid

   #153982

   Purpose

   Express Orf8 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   orf8 (ORF8 Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SARS_COV2_nsp14_HA_BASU

   Plasmid

   #153993

   Purpose

   Express NSP14 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   NSP14 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SARS_COV2_nsp9_HA_BASU

   Plasmid

   #153986

   Purpose

   Express NSP9 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SARS_COV2_nsp5_HA_BASU

   Plasmid

   #153987

   Purpose

   Express NSP5 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   nsp5 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SARS_COV2_N_HA_BASU

   Plasmid

   #153989

   Purpose

   Express N gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SARS_COV2_nsp2_HA_BASU

   Plasmid

   #153988

   Purpose

   Express NSP2 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SARS_COV2_nsp15_HA_BASU

    Plasmid

    #153985

    Purpose

    Express NSP15 gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SARS_COV2_M_HA_BASU

    Plasmid

    #153990

    Purpose

    Express M gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SARS_COV2_ORF7a_HA_BASU

    Plasmid

    #153983

    Purpose

    Express Orf7a gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    orf7a (ORF7a Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-BE3RA-P2A-GFP-PGK-Puro

    Plasmid

    #110868

    Purpose

    Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-HF1RA-PGK-Puro

    Plasmid

    #110860

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid

    #187963

    Purpose

    mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Inserts

    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    osTIR1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-HF1RA-P2A-Puro

    Plasmid

    #110853

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-VQRRA-P2A-Puro

    Plasmid

    #110851

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-VQRRA-P2A-GFP-PGK-Puro

    Plasmid

    #110864

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-VQRRA-PGK-Puro

    Plasmid

    #110858

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDF1-GRM3 (S610L)

    Plasmid

    #31800

    Depositor

    Yardena Samuels

    Article

    Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed serine 610 to leucine

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only