We narrowed to 7,389 results for: ALP

1. pBRα-σ70 D581G

   Plasmid

   #53732

   Purpose

   Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The sigma 70 moiety carries the D581G substitution

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

2. p63RhoGEF619-mVenus-N1

   Plasmid

   #84339

   Purpose

   Expresses p63 (619 a.a.) tagged with mVenus

   Depositor

   Dorus Gadella

   Article

   van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

   Insert

   p63RhoGEF (619 aa) (ARHGEF25 Human)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-Akt1(WT)

   Plasmid

   #86631

   Purpose

   Expresses mCherry-tagged human Akt1(WT)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hRARγ.hRXRα

   Plasmid

   #135416

   Purpose

   Human Retinoic Acid Receptor-gamma and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Inserts

   Human retinoic acid receptor-gamma
   Human retinoid X receptor-alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SV40

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9-GFP_sg_mAMPKa1

   Plasmid

   #79004

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa1 (Prkaa1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1-hRARβ.hRXRα

   Plasmid

   #135415

   Purpose

   Human Retinoic Acid Receptor-beta and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Inserts

   Human retinoic acid receptor-beta
   Human retinoid X receptor-alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SV40

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRII-1.1-2.01 beta

   Plasmid

   #69591

   Purpose

   OT-II TCR beta chain cDNA in pCRII

   Depositor

   Francis R. Carbone

   Article

   Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

   Insert

   TCR beta clone 1.1 (Tcrb Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV6M-Pak1 K299R

   Plasmid

   #12210

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Catalytically inactive Pak1: K299R

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9-GFP_sg_mAMPKa2

   Plasmid

   #79005

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa2 (Prkaa2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pHRSin_hTCRαγεζ

    Plasmid

    #187351

    Purpose

    Lentiviral expression of GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL0341 (pTarget)

    Plasmid

    #200906

    Purpose

    Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    pTarget
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AMPK-γ1-HA

    Plasmid

    #40605

    Depositor

    Harvey Lodish

    Article

    Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

    Insert

    AMPK-γ1 (Prkag1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pEBG-Pak1 83-149

    Plasmid

    #12214

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Dominant negative Pak1: autoinhibitory domain, aa…

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL2783 (pTnsC-VP64_Vch)

    Plasmid

    #200896

    Purpose

    Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsC-VP64
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVenus-N-SLC35B2

    Plasmid

    #155038

    Purpose

    Transient expression of SLC35B2-Venus

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-PH1(WT)

    Plasmid

    #86599

    Purpose

    Expresses eGFP-tagged PH(WT) domain of human Akt1

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 PH domain (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-LAP2-mCherry

    Plasmid

    #159524

    Purpose

    Expresses mCherry from LAP2 promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LAP2

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHRi_1G4 hTCRα

    Plasmid

    #187603

    Purpose

    Lentiviral expression of hTCR alpha (1G4) for low expression

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    1G4 hTCR alpha (TRA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mHSP

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gal4-p160MBP

    Plasmid

    #51

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 Myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    Gal4DBD

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pLAC

    Plasmid

    #45072

    Purpose

    lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide

    Depositor

    Gordon Laurie

    Article

    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

    Insert

    Lacritin (LACRT Human)
    

    Use

    Protein expression vector

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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