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pEYFP-N1-HsSmg6_H

Plasmid

#146475

Purpose

Mammalian Expression of HsSmg6

Depositor

Elisa Izaurralde

Article

Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

Insert

HsSmg6 (SMG6 Human)

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB3405

Plasmid

#106166

Purpose

EasyCloneYALI system-based yeast episomal vector carrying a nourseothricin-resistance marker for Yarrowia lipolytica, can be used for gRNA expression, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMemoryArray

Plasmid

#60585

Purpose

An array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

MemoryArray

Expression

Bacterial

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-BCCP-PupE

Plasmid

#113403

Purpose

Expresses BCCP-PupE in bacteria

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

BCCP-PupE

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pT2HLacZpA1I.7 (#59)

Plasmid

#35965

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter (Tek Mouse)
SDK LacZ
SV40 pA
Tie2 full enhancer

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pIntegrase_3

Plasmid

#60575

Purpose

plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Integrase_3

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pBAD

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTAB182_K

Plasmid

#146777

Purpose

Mammalian Expression of HsTAB182

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTAB182 (TNKS1BP1 Human)

Expression

Mammalian

Mutation

2 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB4586

Plasmid

#106124

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pReporter_3

Plasmid

#60564

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_3

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pReporter_4

Plasmid

#60565

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_4

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEF6a-FKBP-CD28

Plasmid

#113401

Purpose

Expresses FKBP-CD28 in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

FKBP-CD28

Expression

Mammalian

Promoter

EF-1α

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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YFP-DBD

Plasmid

#14874

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

DAG binding domain (Prkcb Rat)

Tags

YFP

Expression

Mammalian

Mutation

Y123W

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pGEX6p-1-FRB-PafA

Plasmid

#113404

Purpose

Expresses FRB-PafA in bacteria

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

FRB-PafA

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HIV-Rev_O

Plasmid

#147113

Purpose

Mammalian Expression of HIV-Rev

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsMago_I

Plasmid

#146579

Purpose

Mammalian Expression of HsMago

Depositor

Elisa Izaurralde

Article

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Insert

HsMago (MAGOH Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pReporter_12

Plasmid

#60572

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_12

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsY14_I

Plasmid

#146580

Purpose

Mammalian Expression of HsY14

Depositor

Elisa Izaurralde

Article

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Insert

HsY14 (RBM8A Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHPyV7-713a

Plasmid

#24728

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

Full genome of Human polyomavirus 7

Use

Viral clone

Available Since

June 8, 2010

Availability

Academic Institutions and Nonprofits only

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pCfB6576

Plasmid

#106134

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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shNg pA_RC3J1_CAGW

Plasmid

#92155

Purpose

Bicistronic AAV construct encoding GFP control and shRNA targeting Ng

Depositor

Weifeng Xu

Article

Han et al Cell Rep. 2017 Jan 24;18(4):892-904. doi

Insert

shRNA targeting Ng (NRGN Human)

Use

AAV

Expression

Mammalian

Promoter

H1

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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