We narrowed to 17,455 results for: emb

1. pclbw-eGFPomp25

   Plasmid

   #58424

   Purpose

   Mammalian expression of GFP targeted to the mitochondrial outer membrane

   Depositor

   David Chan

   Article

   Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

   Insert

   eGFP-omp25 (Synj2bp Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   contains aa's 109-145 of mouse omp25

   Promoter

   CMV/b-actin

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pBTK552

   Plasmid

   #110618

   Purpose

   BTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI.

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pEGFP-Alu-Lin28

   Plasmid

   #92346

   Purpose

   Plasmid for expression of GFP gene with a Lin28 gene derived Alu element containing 3'UTR.

   Depositor

   Gordon Carmichael, Ling-Ling Chen

   Article

   Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

   Insert

   Lin28 gene derived Alu element containing 3'UTR (LIN28A Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-PBD

   Plasmid

   #60880

   Purpose

   Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA365-653

   Depositor

   Trina Schroer

   Article

   Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1

   Insert

   Polo-like kinase 1 (PLK1 Human, BC01486)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pRDB_181

   Plasmid

   #216102

   Purpose

   CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPRv1 sgTollip

   Plasmid

   #196546

   Purpose

   Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

   Depositor

   David Tumbarello

   Article

   Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

   Insert

   Toll-Interacting Protein (TOLLIP Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDA_662

   Plasmid

   #216101

   Purpose

   CRISPRi, EFS-driven dCas9-KRAB (ZNF10) (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDA_831

   Plasmid

   #216100

   Purpose

   CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMHE:mTREK-1(tandem wt-wt)

   Plasmid

   #224757

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1(tandem wt-wt)

   Depositor

   Dan Minor

   Article

   Bagriantsev et al EMBO J. 2012 Aug 1;31(15):3297-308. doi:

   Insert

   Tandem TREK-1 (wt-wt) (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Promoter

   T7

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV10.KH

    Plasmid

    #63208

    Purpose

    pCACS backbone (Amp), Hyg, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5HT6-YFP-ThymosinBeta4(WT)

    Plasmid

    #96806

    Purpose

    cilia-membrane-targeted G-actin-binding protein

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57-CMV-MAAP-SL08

    Plasmid

    #218247

    Purpose

    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein Variant SL08 driven by CMV promoter
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. RHBDD1 pmRFP-N2 (1390)

    Plasmid

    #62049

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    RHBDD1 (RHBDD1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pBTK569

    Plasmid

    #110614

    Purpose

    BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    E2-Crimson
    

    Expression

    Bacterial

    Mutation

    V32M (see comments below)

    Available Since

    Oct. 2, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA5FRT/TO-UBE2QL1-Strep/HA

    Plasmid

    #124666

    Purpose

    Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG MCP-VP64

    Plasmid

    #92357

    Purpose

    CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    MCP-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

    Plasmid

    #129513

    Purpose

    Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 C88S (UBE2QL1 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

    Plasmid

    #188991

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    F163L, K249R, G328S, G381V, G429D, D480G

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

    Plasmid

    #175259

    Purpose

    Dual plasmid labeling membrane target protein of different neuronal population with different fluorophore

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    Lox-rox-STOP-rox-lyn-mScarlet-pA-lox
    

    Use

    Cre/Lox; Dre/rox

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    pCAG

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. RHBDD1 pEGFP-N2 (1078)

    Plasmid

    #62028

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    RHBDD1 (RHBDD1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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