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eOYW007

Plasmid

#238388

Purpose

TurboRFP constitutive Golden Gate construct

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2025 Oct 17;14(10):4122-

Insert

UNS1-P4d-U3d-TurboRFP-t0-UNSX

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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eOYW008

Plasmid

#238389

Purpose

TurboRFP constitutive Golden Gate construct

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2025 Oct 17;14(10):4122-

Insert

UNS1-P4m-U3d-TurboRFP-t0-UNSX

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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eOYW009

Plasmid

#238390

Purpose

TurboRFP constitutive Golden Gate construct

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2025 Oct 17;14(10):4122-

Insert

UNS1-P5d-U3d-TurboRFP-t0-UNSX

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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eOYW012

Plasmid

#238392

Purpose

TurboRFP constitutive Golden Gate construct

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2025 Oct 17;14(10):4122-

Insert

UNS1-P2m-U1d-TurboRFP-t0-UNSX

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJM220-PhoA-mNeongreen-J23101-mScarletI

Plasmid

#240201

Purpose

MiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A strong Anderson promoter (J23101) drives expression of mScarlet-I

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2024 Jan 19;13(1):384-39

Inserts

mNeongreen
mScarlet-I

Promoter

J23101 and PphoA

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJM220-PphoA-mNeongreen-J23116-mScarletI

Plasmid

#240200

Purpose

MiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A weak Anderson promoter (J23116) drives expression of mScarlet-I

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2024 Jan 19;13(1):384-39

Inserts

mNeongreen
mScarlet-I

Promoter

J23116 and PphoA

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1129

Plasmid

#236752

Purpose

BTK Type 2-3 fluorescent protein cassette for golden gate assembly. Improved version of pBTK1055

Depositor

Jeffrey Barrick

Article

Lariviere et al mBio. 2024 Sep 11;15(9):e0139224. doi: 1

Insert

Type 2-3 GFP cassette

Use

Synthetic Biology

Promoter

CP25

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mNeonGreen-G418

Plasmid

#236484

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mNeonGreen

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mStayGold-Hyg

Plasmid

#236486

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mStayGold

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mStayGold-Nat

Plasmid

#236485

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mStayGold

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAP-U2-1

Plasmid

#236472

Purpose

Plasmid for integration at the native URA3 locus with 3xmCherry and 3xGFP under control of the strong bidirectional SpH2A/B promoter.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

spH2Ap-3xmCherry spH2Bp-3xGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mStayGold-G418

Plasmid

#236487

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mStayGold

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mCherry-Nat

Plasmid

#236488

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mCherry

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-sfGFP-Hyg

Plasmid

#236480

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

sfGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mCherry-G418

Plasmid

#236490

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mCherry

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mScarlet-Nat

Plasmid

#236491

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mScarlet

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mCherry-Hyg

Plasmid

#236489

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mCherry

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-Dendra2-G418

Plasmid

#236496

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

Dendra2

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-Nat

Plasmid

#236476

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

GFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-Dendra2-Hyg

Plasmid

#236495

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

Dendra2

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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