Addgene

We narrowed to 21,801 results for: ESC;

Showing: 261 - 280 of 21801 results

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pGL3-Ctr2-gRNA-EGFP

Plasmid

#226001

Purpose

Negative control for CRISPRi-KD

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

negtive control sgRNA of no specific targets in human genome

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-hL1-gRNA1-EGFP

Plasmid

#226002

Purpose

CRISPRi-KD of human LINE1

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human L1HS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-hL1-gRNA2-EGFP

Plasmid

#226003

Purpose

CRISPRi-KD of human LINE1

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human L1HS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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miR290-SE-RGM-tdTomato targeting vector

Plasmid

#133047

Purpose

Targeting vector of RGM-tdTomato with homologous arms around Mir290 super-enhancer DMR

Depositor

Rudolf Jaenisch

Article

Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

Insert

miR290-SE-5arm-RGM-miR290-SE-3arm

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_ISG

Pooled Library

#125753

Purpose

Expresses gRNAs targeting human Interferon-Stimulated Genes

Depositor

Molly OhAinle, Michael Emerman

Article

Roesch et al Retrovirology. 2018 Mar 20;15(1):26. doi

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only
pMuSIC pool v1.0

Pooled Library

#227193

Purpose

Two-way barcodes from 18 fluorescent proteins for mammalian expression. Can be used for clone tracing, or for pairing with perturbations for single cell resolution in spectral detection measurements.

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Expression

Mammalian

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only
plIG-123_HA-GD2-28z_CAR_TF_Library

Pooled Library

#207478

Purpose

HA-GD2-28z CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into th

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-128_HA-GD2-28z_CAR_TFxTF_Library

Pooled Library

#207480

Purpose

HA-GD2-28z CAR in combination with ~10,000 transcription factor combinations (and related proteins) and controls (GFP/RFP combinations). Library can be used to generate HDR templates for modular poole

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-106_NY-ESO-1_TCR_SR_Library

Pooled Library

#207474

Purpose

NY-ESO-1 TCR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-107_NY-ESO-1_TCR_TF_Library

Pooled Library

#207475

Purpose

NY-ESO-1 TCR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-109_CD19-BBz_CAR_SR_Library

Pooled Library

#207476

Purpose

CD19-BBz CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-110_CD19-BBz_CAR_TF_Library

Pooled Library

#207477

Purpose

CD19-BBz CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-124_HA-GD2-28z_CAR_SR_Library

Pooled Library

#207479

Purpose

HA-GD2-28z CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cel

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-132_CD19-28z_CAR_TF_and_SR_Library

Pooled Library

#207481

Purpose

CD19-28z CAR in combination with 129 surface receptors, 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knoc

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
pNR2E3-donor

Plasmid

#176369

Purpose

CRISPR donor plasmid to create GFP fusion proteins

Depositor

Barbara Stranger, Kevin White

Insert

NR2E3 (NR2E3 Human)

Use

CRISPR

Tags

GFP

Expression

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pT2-hUbC-dCas9-KRAB-T2a-Puro

Plasmid

#225999

Purpose

To establish stable dCas9-KRAB knock-in line

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

humanized dCas9-KRAB T2A Puro

Use

Sleeping beauty transposon

Tags

Expression

Mammalian

Mutation

D10A and H840A

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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OCT4 GFP puro Donor 1

Plasmid

#22209

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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TV-hPITX3-forward

Plasmid

#22073

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

PITX3 (ASMD Human)

Use

Cre/Lox; Zfn donor

Tags

eGFP

Expression

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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OCT4 GFP puro Donor 2

Plasmid

#22210

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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OCT4 GFP puro Donor 3

Plasmid

#22211

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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