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Plasmid#154010PurposeExpresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorInsertGSK3B (GSK3B Human)
UseTagsHA tag, His6, and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
Plasmid#83485PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A_R1048A_H1053A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053APromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-MBP-TEV-GG
Plasmid#215800PurposeCloning of target gene with N terminal His6 MBP tag and TEV CleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pH6-MBP-TEV-BirA
Plasmid#179694PurposeExpresses His6 tagged MBP-BirA fusion proteinDepositorInsertBirA
UseTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-NLS-Geo_st
Plasmid#87703PurposeExpression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLSDepositorInsertGeoCas9
UseTags10xHis-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-MBP-4xDQNAT
Plasmid#128398PurposePeriplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertMaltose binding protein
UseTags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationY342H- please see depositor commentsPromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-Cdc42-Q61L
Plasmid#214288PurposeExpress constitutively active Cdc42 fused with MBPDepositorInsertConstitutively active Cdc42 (CDC42 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationCdc42-Q61LPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_WT
Plasmid#83482PurposeBacterial expression of WT L. buccalis C2c2 CRISPR effector.DepositorInsertLbu_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MBP-2xNLS-tdTomato
Plasmid#104054PurposeAn AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoterDepositorInsert2xNLS-tdTomato
UseAAVTagsNLSExpressionMutationPromoterMBPAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-TSF-TEVcs-ULK1
Plasmid#171416PurposeFor the purifcation of human ULK1 complexDepositorInsertULK1 (ULK1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-xMBP-ACE2-CHis
Plasmid#194998PurposeProduction of ACE2 extracellular domainDepositorInsertACE2 (ACE2 Human)
UseTagsHis8 and xMBPExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET_MBP-RGG-GFP-RGG
Plasmid#124949PurposeExpression of MBP-RGG-GFP-RGGDepositorInsertMBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsEGFP, His, and MBPExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pES002 MBP-GSK3β_S9A-HA-His
Plasmid#196184PurposeExpresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorInsertGSK3B (GSK3B Human)
UseTagsHA, His6, and MBPExpressionBacterialMutationchanged Serine 9 to AlaninePromoterTacAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6His-MBP-MpAgo
Plasmid#79249PurposeThis plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expressionDepositorInsertMarinitoga piezophila Argonaute
UseCRISPRTags6XHis-tag + MBP + PreScission Cleavage SiteExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MBP-His-ACPs
Plasmid#141140PurposeExpression of holo-[acyl-carrier-protein] synthaseDepositorInsertacpS
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceMay 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET20b-NLuc-MBP-6xHis
Plasmid#135931PurposeExpresses NanoLuciferase in bacterial cells.DepositorInsertNanoLuciferase
UseTagsMBP-6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
Plasmid#222991PurposeBacterial expression of iGeoCas9(C2) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogTag-MBP
Plasmid#171773PurposeExpresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogTag-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)/MBP-His
Plasmid#38006DepositorInsertMBP (Maltose binding protein)
UseTags6xHISExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lba_Cas13a_WT
Plasmid#91867PurposeBacterial expression for Cas13aDepositorInsertLbaCas13a (LbaC2c2) WT
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only