We narrowed to 11,747 results for: SHA

1. pQP-2473

   Plasmid

   #138976

   Purpose

   Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQP-CiRISB

   Plasmid

   #138978

   Purpose

   Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQP-AR10

   Plasmid

   #138980

   Purpose

   Endogenous RAS regulation using light-controlled intrabody

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQP-NiRISB

   Plasmid

   #138977

   Purpose

   Light-controlled tri-directional targeting of endogenous proteins (nucleus-shifted)

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1-CAAX-IRES2-NES- iB(GFP)- mCherry-Q-PAS1-AsLOV2cNLS-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQP-iRISB

   Plasmid

   #138979

   Purpose

   Light-controlled tri-directional targeting of endogenous proteins

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQP-2363

   Plasmid

   #138975

   Purpose

   Relocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illumination

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-V5-DGCR8

   Plasmid

   #51383

   Depositor

   Narry Kim

   Article

   Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

   Insert

   DGCR8 (DGCR8 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS156

   Plasmid

   #182707

   Purpose

   CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

   Depositor

   Rebecca Shapiro

   Article

   Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQP-2460

   Plasmid

   #138973

   Purpose

   Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

   Depositor

   Vladislav Verkhusha

   Article

   Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

   Insert

   NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSETC-PITPbeta (splice variant 1)

    Plasmid

    #58270

    Purpose

    bacterial expression of PITP beta (phosphatidylinositol transfer protein beta)

    Depositor

    Shamshad Cockcroft

    Article

    Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

11. NmMetNI-WT

    Plasmid

    #172114

    Purpose

    Expression of MetN and MetI membrane proteins

    Depositor

    Douglas Rees

    Article

    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:

    Inserts

    MetN
    MetI
    

    Use

    Tags

    10x His Tag and Enterokinase

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQP-2376

    Plasmid

    #138974

    Purpose

    Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSETC-PITPbeta (splice variant 1) K61A

    Plasmid

    #58271

    Purpose

    bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

    Depositor

    Shamshad Cockcroft

    Article

    Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pLV-mScarlet3-CAAX-IRES-Puro

    Plasmid

    #222639

    Purpose

    Live-cell plasma membrane marker

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mScarlet3-CAAX
    

    Use

    Lentiviral

    Tags

    mScarlet3

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-RhoB Sensor-IRES-Blast

    Plasmid

    #222633

    Purpose

    Rho reporter

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    RhoB Sensor
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-V5-DGCR8-mDRBD1&2

    Plasmid

    #51385

    Depositor

    Narry Kim

    Article

    Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

    Insert

    DGCR8 (DGCR8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    DRBD1 & DRBD2 mutant (A568K, A569K, A676K, S6…

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-V5-DGCR8-mDRBD1

    Plasmid

    #51384

    Depositor

    Narry Kim

    Article

    Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

    Insert

    DGCR8 (DGCR8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    DRBD1 mutant (A568K, A569K)

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-V5-DGCR8-mDRBD2

    Plasmid

    #51386

    Depositor

    Narry Kim

    Article

    Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

    Insert

    DGCR8 (DGCR8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    DRBD2 mutant (A676K, S677K)

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pNEU-KlacRS

    Plasmid

    #232155

    Purpose

    Site-specific incorporation of L-lactyl-lysine into target proteins in mammalian cells.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    KlacRS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-mCherry-TAG-EGFP

    Plasmid

    #232156

    Purpose

    A fluorescence reporter to show incorporation of target unnatural amino acids.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    mCherry-linker(TAG)-EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge